Expression of monocyte chemoattractant protein-1 in rat dorsal root ganglia and spinal cord in experimental models of neuropathic pain
Expression of monocyte chemoattractant protein-1 in rat dorsal root ganglia and spinal cord in experimental models of neuropathic pain
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DOI：
10.1016/j.brainres.2008.11.046
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发表时间：
2009-01-28
期刊：
影响因子：
2.9
通讯作者：
Cho, Hee-Jung
中科院分区：
文献类型：
--
作者：
Jeon, Sang-Min;Lee, Kyung-Min;Cho, Hee-Jung
在这项研究中，我们评估了MCP-1的表达在大鼠背根神经节（DRG）和脊髓轴突切断和慢性压迫性损伤（CCI）的坐骨神经和L5脊神经结扎（L5 SNL），使用免疫组化方法。MCP-1在DRG中的表达在神经损伤后疼痛超敏反应完全发作之前达到峰值并下降。当机械性异常性疼痛最大时，MCP-1的脊髓表达达到峰值，但随后迅速下降，尽管机械性异常性疼痛显著持续。结果表明，MCP-1可能参与神经病理性疼痛的启动，而不是在其维持。尽管MCP-1在小的和大的DRG神经元中增加，但在CCI和L5 SNL后的脊髓浅层中观察到MCP-1-IR终末显著增加，但在轴突切断后没有观察到MCP-1-IR终末;然而，在深层中，在L5 SNL后发现MCP-1-IR终末显著增加，其可能来源于大的和受损的L5 DRG神经元。我们的研究结果表明，MCP-1在DRG神经元合成可能会或可能不会被运送到脊髓取决于周围神经损伤的类型。此外，完整的L4和受损的L5 DRG神经元中MCP-1的增加可能有助于L5 SNL后的神经性疼痛超敏反应。(c)2008 Elsevier B. V.保留所有权利。
In this study, we evaluated the expression of MCP-1 in the rat dorsal root ganglion (DRG) and spinal cord following axotomy and chronic constriction injury (CCI) of the sciatic nerve and L5 spinal nerve ligation (L5 SNL) using an immunohistochemical approach. MCP-1 expression in the DRG peaked and declined before the full onset of pain hypersensitivity following nerve injury. Spinal expression of MCP-1 peaked when mechanical allodynia was maximal, but then declined rapidly despite the remarkable persistence of mechanical allodynia. The results suggest that MCP-1 may participate in the initiation of neuropathic pain, rather than in its maintenance. Despite increased MCP-1 in small and large DRG neurons, a remarkable increase in MCP-1-IR terminals was observed in the spinal superficial laminae following CCI and L5SNL, but not following axotomy; however, in the deeper laminae, a considerable increase in MCP-1-IR terminals, which may originate from the large and injured L5 DRG neurons, was found after L5 SNL. Our results demonstrate that MCP-1 synthesized in DRG neurons may or may not be transported to the spinal cord depending on the type of peripheral nerve injury. Additionally, increased MCP-1 in both intact L4 and injured L5 DRG neurons may contribute to neuropathic pain hypersensitivity following L5 SNL. (c) 2008 Elsevier B.V. All rights reserved.