Modification, characterization and structure–anticoagulant activity relationships of persimmon polysaccharides
Modification, characterization and structure–anticoagulant activity relationships of persimmon polysaccharides
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DOI：
10.1016/j.carbpol.2010.05.008
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发表时间：
2010-09
影响因子：
11.2
通讯作者：
Ya-li Zhang;Jianbao Zhang;Xiaoyan Mo;Xiaoyun Lu;Yun-feng Zhang;Liguo Qin
中科院分区：
文献类型：
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作者：
Ya-li Zhang;Jianbao Zhang;Xiaoyan Mo;Xiaoyun Lu;Yun-feng Zhang;Liguo Qin
以新鲜柿果为原料，采用氯磺酸-吡啶法和超声波降解法制备了6种不同取代度（DS）和分子量（Mw）的化学硫酸酯化多糖衍生物（PFP-S），并通过DS、FT-IR和电位分析对反应效果进行了表征。硫酸化改性可引起样品的高级结构变化，PFP-S的扫描电镜显示出多相卷曲结构。抗凝活性测定结果表明，PFP-S能延长活化部分凝血活酶时间（APTT）和凝血酶时间（TT），但对凝血酶原时间（PT）无影响，且随着取代度的增加和分子量的降低，PFP-S的抗凝活性增强。
Six chemical sulfated polysaccharide derivatives (PFP-S) with variable degree of substitution (DS) and molecular weights (Mw) were prepared from the fresh persimmon fruits by chlorosulfonic acid-pyridine and ultrasonic degradation methods, and the effectiveness of the reaction was monitored by the DS, FT-IR and ζ-potential analysis. The sulfation modification might cause advanced structure change to the samples, the SEM of PFP-S exhibited a polyphasic convoluted structure. Results of anticoagulant activities showed that PFP-S could prolong activated partial thromboplastin time (APTT) and thrombin time (TT), but not prothrombin time (PT), and the anticoagulant activity improved with the increasing DS and decreasing Mw.