TRANSIENT CONFORMATION CHANGES IN CHROMATIN DURING EXCISION REPAIR OF ULTRAVIOLET DAMAGE TO DNA
TRANSIENT CONFORMATION CHANGES IN CHROMATIN DURING EXCISION REPAIR OF ULTRAVIOLET DAMAGE TO DNA
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DOI：
10.1093/nar/9.1.203
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发表时间：
1981-01-01
影响因子：
14.9
通讯作者：
CLEAVER, JE
中科院分区：
文献类型：
--
作者：
BODELL, WJ;CLEAVER, JE
在紫外线诱导的修复合成过程中标记15分钟的DNA对微球菌核酸酶的敏感性是大块核DNA的两倍。随着标记时间从15分钟增加到4小时，修复标记DNA的核酸酶敏感性接近于整体染色质。脉冲追逐实验表明，在短脉冲期间标记的修复DNA的核酸酶敏感性降低，半衰期约为15分钟。与之前的解释不同，我们认为这些结果意味着在合成后立即，在修复过程中标记的染色质具有一种构象，使其比整体染色质更容易被核酸酶消化。随着时间的推移，这些修复的区域被组装成具有正常核酸酶敏感性的核小体结构。
DNA labeled for 15 minutes during UV induced repair synthesis is two-fold more sensitive to micrococcal nuclease than the bulk nuclear DNA. As the length of the labeling period increases from 15 minutes to 4 hours the nuclease sensitivity of repair labeled DNA approaches that of bulk chromatin. Pulse-chase experiments indicate that the nuclease sensitivity of the repaired DNA labeled during a brief pulse decreases with a half-life of about 15 minutes. In contrast to previous interpretations, we consider these results to mean that immediately after synthesis, chromatin labeled during repair has a conformation which renders it more susceptible to nuclease digestion than the bulk chromatin. With time these repaired regions are assembled into a nucleosome structure with normal nuclease sensitivity.