SYNTHESIS OF 4R-TRITIUM AND 4S-TRITIUM LABELED NADPH FOR THE DETERMINATION OF THE COENZYME STEREOSPECIFICITY OF NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE
SYNTHESIS OF 4R-TRITIUM AND 4S-TRITIUM LABELED NADPH FOR THE DETERMINATION OF THE COENZYME STEREOSPECIFICITY OF NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE
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DOI：
10.1016/0006-291x(87)91141-7
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发表时间：
1987-10-14
影响因子：
3.1
通讯作者：
RICHARDS, WR
中科院分区：
文献类型：
--
作者：
VALERA, V;FUNG, M;RICHARDS, WR
本文介绍了一种快速简便地制备4 R和4S氚标记的NADH或NADPH异构体的方法。该方法仅需要使用单一标记化合物（D-（1- 3 H）葡萄糖）和两种酶（来自芽孢杆菌属的葡萄糖脱氢酶和来自布氏嗜热厌氧菌的醇脱氢酶），这两种酶分别对NADH或NADPH的pro S和pro R氢具有特异性。用~（4 R）和~（4S）氚标记的NADPH异构体测定了黄化小麦中NADPH：原叶绿素氧化还原酶对NADPH前S氢的专一性。
A rapid and easy method for the production of both the 4R and 4S tritium labeled isomers of either NADH or NADPH has been developed. The method requires the use of only a single labeled compound (D-(1-3H)glucose), and two enzymes (glucose dehydrogenase from Bacillus sp. and alcohol dehydrogenase from Thermoanaerobium brockii) which, are specific for the pro S and pro R hydrogens, respectively, of either NADH or NADPH. The 4R and 4S tritium labeled isomers of NADPH have been used to determine that NADPH:protochlorophyllide oxidoreductase from etiolated wheat was specific for the pro S hydrogen of NADPH.