ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .34. TERMINATION OF CHAIN GROWTH BY A 2',3'-DIDEOXYRIBONUCLEOTIDE
ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID .34. TERMINATION OF CHAIN GROWTH BY A 2',3'-DIDEOXYRIBONUCLEOTIDE
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DOI：
10.1021/bi00840a037
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发表时间：
1969-01-01
期刊：
影响因子：
2.9
通讯作者：
MOFFATT, JG
中科院分区：
文献类型：
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作者：
ATKINSON, MR;DEUTSCHER, MP;MOFFATT, JG
摘要：2‘，3’-双脱氧核糖核苷三磷酸类似于脱氧核糖核酸聚合酶的天然2‘-脱氧核糖核苷酸底物，但缺乏脱氧核糖核酸链生长所需的3’-羟基。双脱氧核苷酸的结合阻止了脱氧核糖核酸的合成，并抑制了发生在来自大肠杆菌的脱氧核糖核酸聚合酶的引物位置的相关反应(焦磷酸分解、焦磷酸交换和水解)。链末端的双脱氧胸苷基团与脱氧核糖核酸和寡聚脱氧核糖核酸链的结合约为1000
Maurice R. Atkinson, f Murray P. Deutscher, J Arthur Kornberg, Alan F. Russell, § and J. G. Moffatt abstract: 2', 3'-Dideoxyribonucleoside triphosphates are analogs of the natural 2'-deoxyribonucleotide substrates of deoxyribonucleic acid polymerase but lack the 3'-hydroxyl group required for deoxyribonucleic acid chain growth. Attachment of a dideoxynucleotide blocks deoxyribonucleic acid synthesis and inhibits related reactions (pyrophosphorol-ysis, pyrophosphate exchange, and hydrolysis) which occur at the primer site of deoxyribonucleic acid polymerase from Escherichia coli. Attachment of a chain-terminating dideoxy-thymidylate group to deoxyribonucleic acid andto oligo-and polydeoxynucleotide chains is approximately a thousand