Origin of tubular complexes developing during induction of pancreatic adenocarcinoma by 7,12-dimethylbenz(a)anthracene.
Origin of tubular complexes developing during induction of pancreatic adenocarcinoma by 7,12-dimethylbenz(a)anthracene.
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7,12-二甲基苯并(a)蒽诱导胰腺腺癌过程中管状复合物的起源。
DOI：
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发表时间：
1978
影响因子：
6
通讯作者：
P. D. Webster
中科院分区：
文献类型：
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作者：
D. Bockman;O. Black;L. R. Mills;P. D. Webster
将 7,12-二甲基苯并(a)蒽 (DMBA) 植入大鼠胰腺已被证明可诱发腺癌。具有增殖的小叶内导管外观的小管复合体经常出现在肿瘤发展过程中。通过光学和电子显微镜研究这些复合物以确定它们的形成方法。此外，从连续切片重建管状复合体以确定其三维构型。尽管其他人认为管状复合体是由导管增殖引起的，但以下观察表明它们起源于含有酶原颗粒的细胞：a）腺泡和肾小管之间存在连续的过渡阶段，b）大多数肾小管尺寸减小并被结缔组织取代（退化而不是增殖的证据），c）管状复合体中几乎没有看到有丝分裂图，d） 小管由许多细胞组成，这些细胞具有丰富的粗面内质网，这是一种导管稀疏的细胞器，并且 e) 小管的三维排列看起来与正常大鼠胰腺中含有酶原颗粒的细胞的分支、吻合排列相同。仅在胰腺中放置缝合线的对照动物表现出最小的反应。结论是，当酶原颗粒的损失伴随 DMBA 诱导肿瘤时，“腺泡”被识别为小管。这些细胞的转化可能被错误地解释为增殖导管的转化。
Implantation of 7,12-dimethylbenz(a)anthracene (DMBA) into the pancreas of rats has been shown to induce adenocarcinoma. Complexes of tubules, which have the appearance of proliferated intralobular ducts, frequently appear during tumor development. These complexes were studied by light and electron microscopy to determine their method of formation. In addition, a tubular complex was reconstructed from serial sections to determine its three-dimensional configuration. Although tubular complexes have been thought by others to result from ductal proliferation, the following observation indicate that they originate from zymogen-granule-containing cells: a) there is a continuum of transitional stages between acini and tubules, b) most tubules decrease in size and are replaced by connective tissue (evidence of regression rather than proliferation), c) few mitotic figures are seen in tubular complexes, d) the tubules comprise many cells which have an abundance of rough endoplasmic reticulum, an organelle which is sparce in ducts, and e) the three-dimensional arrangement of tubules appears identical to the branching, anastomosing arrangement of zymogen-granule-containing cells of the normal rat pancreas. Control animals in which only sutures were placed in the pancreas showed minimal reaction. It is concluded that "acini" become recognized as tubules when loss of zymogen granules accompanies tumor induction by DMBA. Transformation of these cells could be erroneously interpreted as transformation from proliferating ducts.