Development and characterization of a model system to study amphibian immune responses to iridoviruses
Development and characterization of a model system to study amphibian immune responses to iridoviruses
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DOI：
10.1016/s0042-6822(03)00151-x
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发表时间：
2003-07-05
期刊：
影响因子：
3.7
通讯作者：
Robert, J
中科院分区：
文献类型：
--
作者：
Gantress, J;Maniero, GD;Robert, J
最近认识到虹彩病毒科的病毒可能导致世界范围内两栖动物的减少，这使得了解两栖动物的抗病毒免疫防御变得迫切。我们目前的证据表明，建立青蛙非洲爪蟾作为一个重要的模型，研究抗虹彩病毒免疫。成年人抵抗高剂量的FV 3感染，仅显示出短暂的病理迹象。相比之下，天然MHC I类缺陷的蝌蚪对FV 3感染高度敏感。通过PCR监测病毒DNA表明FV 3 DNA在肾脏中的优先定位，近交系MHC纯合J株似乎更易感。通过检测FV 3 DNA测量的病毒清除以及感染的病理和行为症状的消失、病毒清除的加速和第二次注射FV 3后IgY抗-FV 3抗体的检测都与细胞和体液适应性抗病毒免疫应答的参与一致。(C)2003 Elsevier Science（美国）。All rights reserved.
The recent realization that viruses within the family Iridoviridae may contribute to the worldwide decline in amphibians makes it urgent to understand amphibian antiviral immune defenses. We present evidence that establishes the frog Xenopus laevis as an important model with which to study anti-iridovirus immunity. Adults resist high doses of FV3 infection, showing only transitory signs of pathology. By contrast, naturally MHC class-I-deficient tadpoles are highly susceptible to FV3 infection. Monitoring of viral DNA by PCR indicates a preferential localization of FV3 DNA in the kidney, with the inbred MHC homozygous J strain appearing to be more susceptible. Clearance of virus as measured by detection of FV3 DNA and also the disappearance of pathological and behavioral symptoms of infection, acceleration of viral clearance, and detection of IgY anti-FV3 antibodies after a second injection of FV3 are all consistent with the involvement of both cellular and humoral adaptive antiviral immune responses. (C) 2003 Elsevier Science (USA). All rights reserved.