A fluorescence "turn-on" sensor for detecting hydrazine in environment
A fluorescence "turn-on" sensor for detecting hydrazine in environment
复制标题
用于检测环境中肼的荧光“开启”传感器
DOI：
10.1016/j.microc.2019.104376
复制
发表时间：
2020-01-01
影响因子：
4.8
通讯作者：
Hou Linxi
中科院分区：
文献类型：
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作者：
Jiang Xingzong;Zhen, Lu;Hou Linxi
肼（N2H4）因其高毒性已被列为环境污染物和人类致癌物。暴露在高水平的联氨中会对人体造成不可逆转的损害。因此，探索一种有效的方法对其进行选择性识别和检测具有重要意义。本文以5-氯噻吩-2-羰基氯为电子给体，香豆素为电子受体，基于PET（光诱导电子转移）机理，设计了一种新的“开启”荧光探针香豆素-噻吩（C-T）。用联氨处理后，剥离5-氯噻吩-2-羰基氯，可恢复因PET中断而产生的荧光。通过H-1核磁共振滴定和光谱分析进一步验证了其作用机理。此外，该探针对肼的选择性高于其他常见离子和含胺物质，在450 nm处具有明显的荧光增强。该探针可以在较宽的pH范围（4-10）内工作，检出限低至0.0047 μ M (1.5 ppb)，并且可以选择性地检测不同水样中的联氨，具有实际应用潜力。
Hydrazine (N2H4) has been classified as an environmental contaminant and human carcinogen owing to its high toxicity. Being exposed to high levels of hydrazine can cause irreversible damage to human bodies. Hence, it is significant to explore an effective method to selectively recognize and detect it. In this work, a new "turn-on" fluorescence probe, coumarin-thiophene (C-T), was designed based on PET (photo-induced electron transfer) mechanism by employing 5-chlorothiophene-2-carbonyl chloride as a electron donor and coumarin as a electron acceptor. The flourescence could be recovered due to the interruption of PET by stripping the 5-chlorothiophene-2-carbonyl chloride when treated with hydrazine. The mechanism was further verified through H-1 NMR titration and spectral analysis. Additionally, The probe exhibited a high selectivity towards hydrazine over other common ions and amine-containing species with a distinct fluorescence enhancement at 450 nm. The probe could operate over a wide pH region (4-10) with a detection limit as low as 0.0047 mu M (1.5 ppb), Furthermore, it could selectively detect hydrazine in different water samples which indicated its potential for practical applications.