Discovery of tear biomarkers in children with chronic non-infectious anterior uveitis: a pilot study
Discovery of tear biomarkers in children with chronic non-infectious anterior uveitis: a pilot study
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DOI：
10.1186/s12348-018-0156-5
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发表时间：
2018-10-16
影响因子：
2.9
通讯作者：
Holland, Gary N.
中科院分区：
文献类型：
--
作者：
Angeles-Han, Sheila T.;Yeh, Steven;Holland, Gary N.
背景：需要在容易获得的标本中找到生物标志物，以准确预测幼年特发性关节炎（JIA）患儿的葡萄膜炎。房水已被用于研究生物标志物，但不是常规可用的。我们评估了慢性前葡萄膜炎（CAU）儿童的泪液中报告的房水生物标志物。在这项初步研究中，我们使用Schirmer试纸收集了7名儿童（9只眼睛）的眼泪；JIA相关性葡萄膜炎（JIA- u） 3例，特发性葡萄膜炎（I-CAU） 4例。采用液相色谱-串联质谱法对泪液蛋白进行鉴定和定量。Mann-Whitney U检验发现JIA-U患儿和I-CAU患儿泪液蛋白表达差异。结果所有患儿泪液中均检测到ss100a9、LAP3、TTR、MIF、sCD14、S100A8、SAA1；同样的细胞因子在JIA-U患儿房水中也有报道。JIA-U患儿的眼泪中炎性关节炎相关蛋白（SEMA3G、TIMP1、HEXB、ERN1和SAA1）的表达高于I-CAU患儿的眼泪。此外，我们发现，与I-CAU患儿的眼泪相比，JIA-U患儿的眼泪中sCD14、S100A8和SAA1的表达较高，而S100A9、LAP3、TTR和MIF的表达较低。结论肝细胞因子与房水在CAU患儿中具有相似的细胞因子谱，可能是临床有用的疾病生物标志物来源。JIA-U患儿的眼泪中也含有与炎性关节炎相关的细胞因子；此外，其他泪液蛋白的差异表达也可能为JIA-U和I-CAU之间的内在差异提供线索，尽管它们的临床表型相似。
BackgroundBiomarkers in easily obtained specimens that accurately predict uveitis in children with juvenile idiopathic arthritis (JIA) are needed. Aqueous humor has been studied for biomarkers, but is not routinely available. We evaluated tears from children with chronic anterior uveitis (CAU) for biomarkers reported in aqueous humor.In this pilot study, we used Schirmer strips to collect tears from seven children (nine eyes); three children had JIA- associated uveitis (JIA-U) and four had idiopathic disease (I-CAU). Liquid chromatography-tandem mass spectrometry was used to identify and quantify tear proteins. The Mann-Whitney U test identified differential tear protein expression between children with JIA-U and those with I-CAU.ResultsS100A9, LAP3, TTR, MIF, sCD14, S100A8, and SAA1 were detected in tears of all children; the same cytokines have been reported in aqueous humor of children with JIA-U. Tears from children with JIA-U had higher expression of proteins associated with inflammatory arthritis (SEMA3G, TIMP1, HEXB, ERN1, and SAA1) than tears from those with I-CAU. In addition, we found higher expression of sCD14, S100A8, and SAA1, but lower expression of S100A9, LAP3, TTR, and MIF, in tears from children with JIA-U compared to tears from those with I-CAU.ConclusionsTears contain similar cytokine profiles to aqueous humor in children with CAU and may be a clinically useful source of disease biomarkers. Tears from children with JIA-U also contain cytokines associated with inflammatory arthritis; furthermore, differential expression of other tear proteins as well may provide clues to intrinsic differences between JIA-U and I-CAU, despite their similar clinical phenotypes.