Individual pineal cells exhibit a circadian rhythm in melatonin secretion.
Individual pineal cells exhibit a circadian rhythm in melatonin secretion.
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单个松果体细胞在褪黑激素分泌方面表现出昼夜节律。
DOI：
10.1016/0006-8993(93)90757-e
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Tang,WX
中科院分区：
文献类型：
--
作者：
Pickard,GE;Tang,WX
松果体和分离的松果体细胞在体外表现出褪黑激素分泌的昼夜节律，在恒定条件下持续几个周期。目前还不知道是否个别，物理隔离的松果体细胞能够产生昼夜振荡褪黑激素的释放。利用反向溶血空斑试验检测单个松果体细胞的褪黑激素分泌，解决了这个问题。从变色龙蜥蜴，保持在短期文化中，分离的松果体细胞，表现出显着的变化，褪黑激素分泌长达72小时，在循环照明条件下，并在恒定的黑暗。持续的日常波动的褪黑激素分泌从个别细胞强烈表明，个别松果体细胞可以作为昼夜节律振荡器。
Pineal glands and dissociated pineal cells exhibit a circadian rhythm of melatonin secretion in vitro which persists for several cycles under constant conditions. It is not known whether individual, physically isolated pineal cells are capable of generating a circadian oscillation in melatonin release. This question was addressed by utilizing a reverse hemolytic plaque assay for the detection of melatonin secretion from individual pineal cells. Dissociated pineal cells from the anole lizard, maintained in short term culture, displayed a marked variation in melatonin secretion for up to 72 h under both cyclic lighting conditions and in constant dark. The persistence of daily fluctuations of melatonin secretion from individual cells strongly suggests that individual pineal cells can function as circadian oscillators.