A serum ferritin assay for prevalence studies of iron deficiency
A serum ferritin assay for prevalence studies of iron deficiency
复制标题
用于缺铁患病率研究的血清铁蛋白测定
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
J. Cook
中科院分区：
文献类型：
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作者：
C. Flowers;M. Kuizon;J. Beard;B. Skikne;A. Covell;J. Cook
一种专门的血清铁蛋白测定已被开发用于流行病学研究中铁缺乏的检测。采用酶免疫测定法（EIA）来消除对放射性同位素的需求。单克隆免疫试剂的使用消除了血清铁蛋白EIA固有的灵敏度低的问题。方法的工作范围为1-100 μg/L，灵敏度为0.5 μg/ L。在本方法和双位点免疫放射分析（IRMA）之间观察到血清铁蛋白水平的良好一致性，而EIA在低铁蛋白浓度下的变异性显著较小。由于每次测定仅需10 μl血清，因此可在单个血液毛细管上进行重复测量。当使用自动微量滴定板读数器进行光密度测量时，一名技术人员可以在一个工作日内完成80 - 100次重复测定。
A specialized serum ferritin assay has been developed for the detection of iron deficiency in epidemiologic studies. An enzyme immunoassay (EIA) was employed to climinate the need for radioisotopes. The problem of low sensitivity inherent with the EIA for serum ferritin was eliminated by the use of monoclonal immunologic reagents. The working range of the assay is 1–100 μg/L with a sensitivity of 0.5 μg/ L. Excellent agreement in serum ferritin levels was observed between the present method and the two‐site immunoradiometric assay (IRMA), while the variability at low ferritin concentrations was significantly less with the EIA. Because only 10 μl of serum is required for each assay, duplicate measurements can be performed on a single capillary tube of blood. When an automatic microtiter plate reader for optical density measurements is used, 80‐100 duplicate determinations can be completed by one technologist in a single working day.