Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration.
Possible Involvement of Smad Signaling Pathways in Induction of Odontoblastic Properties in KN-3 Cells by Bone Morphogenetic Protein-2: A Growth Factor to Induce Dentin Regeneration.
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DOI：
10.1155/2012/258469
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发表时间：
2012
影响因子：
2.1
通讯作者：
Nishihara T
中科院分区：
文献类型：
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作者：
Washio A;Kitamura C;Morotomi T;Terashita M;Nishihara T
研究骨形态发生蛋白-2(BMP-2)对成牙本质细胞样细胞KN-3生长、分化及细胞内信号转导途径的影响，以阐明成牙本质细胞在牙髓再生过程中分化的分子机制。用BMP-2处理后，观察细胞形态、生长、碱性磷酸酶(ALP)活性、BMP诱导的细胞内信号分子Smad1/5/8和Smad6/7的激活和表达以及牙本质涎蛋白(DSP)和牙本质基质蛋白1(DMP1)的活性。BMP-2对KN-3细胞的形态、生长及碱性磷酸酶活性无影响，但可诱导Smad1/5/8的磷酸化和Smad6/7的表达。BMP-2还可诱导DSP和DMP-1的表达。我们的结果提示，BMP-2可能通过激活Smad信号通路来表达成牙本质细胞表型。
We examined the effects of bone morphogenetic protein-2 (BMP-2) on growth, differentiation, and intracellular signaling pathways of odontoblast-like cells, KN-3 cells, to clarify molecular mechanisms of odontoblast differentiation during pulp regeneration process. After treatment with BMP-2, the cell morphology, growth, alkaline phosphatase (ALP) activity, and the activation and expression of BMP-induced intracellular signaling molecules, such as Smad1/5/8 and Smad6/7, as well as activities of dentin sialoprotein (DSP) and dentin matrix protein 1 (DMP1), were examined. BMP-2 had no effects on the morphology, growth, or ALP activity of KN-3 cells, whereas it induced the phosphorylation of Smad1/5/8 and expression of Smad6/7. BMP-2 also induced the expressions of DSP and DMP-1. Our results suggest that KN-3 cells may express an odontoblastic phenotype with the addition of BMP-2 through the activation of Smad signaling pathways.