ExoCarta: A Web-Based Compendium of Exosomal Cargo.
ExoCarta: A Web-Based Compendium of Exosomal Cargo.
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DOI：
10.1016/j.jmb.2015.09.019
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发表时间：
2016-02-22
影响因子：
5.6
通讯作者：
Mathivanan S
中科院分区：
文献类型：
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作者：
Keerthikumar S;Chisanga D;Ariyaratne D;Al Saffar H;Anand S;Zhao K;Samuel M;Pathan M;Jois M;Chilamkurti N;Gangoda L;Mathivanan S
外泌体是由多种细胞释放到细胞外微环境中的膜囊泡，并参与细胞间通讯。由于外泌体含有RNA、蛋白质和脂质，因此对表征外泌体的分子货物存在重大兴趣。在这里，我们描述了ExoCarta（http：www.exocarta.org），一个手动策划的基于网络的外泌体蛋白、RNA和脂质的纲要。自建立以来，该数据库的访问量很大（来自135个国家的54 000多名访问者）。目前版本的ExoCarta包含来自超过286项外泌体研究的41，860种蛋白质，> 7，540种RNA和1，116种脂质分子，这些外泌体研究注释了ISEV定义细胞外囊泡的最低实验要求。此外，ExoCarta还具有动态蛋白质-蛋白质相互作用网络和外泌体蛋白的生物学途径。用户可以根据研究数量下载最常识别的外泌体蛋白。下载的文件可以进一步直接导入FunRich（http：www.funrich.org）工具，以进行额外的功能丰富和交互网络分析。
Exosomes are membranous vesicles that are released by a variety of cells into the extracellular microenvironment and are implicated in intercellular communication. As exosomes contain RNA, proteins and lipids, there is a significant interest in characterizing the molecular cargo of exosomes. Here, we describe ExoCarta (http://www.exocarta.org), a manually curated web-based compendium of exosomal proteins, RNAs and lipids. Since its inception, the database has been highly accessed (>54,000 visitors from 135 countries). The current version of ExoCarta hosts 41,860 proteins, >7,540 RNA and 1,116 lipid molecules from more than 286 exosomal studies annotated with ISEV minimal experimental requirements for definition of extracellular vesicles. Besides, ExoCarta features dynamic protein-protein interaction networks and biological pathways of exosomal proteins. Users can download most often identified exosomal proteins based on the number of studies. The downloaded files can further be imported directly into FunRich (http://www.funrich.org) tool for additional functional enrichment and interaction network analysis.