DELAY OF TOMATO FRUIT RIPENING BY AN OLIGOSACCHARIDE N-GLYCAN - INTERACTIONS WITH IAA, GALACTOSE AND LECTINS
DELAY OF TOMATO FRUIT RIPENING BY AN OLIGOSACCHARIDE N-GLYCAN - INTERACTIONS WITH IAA, GALACTOSE AND LECTINS
复制标题
DOI：
10.1111/j.1399-3054.1994.tb02205.x
复制
发表时间：
1994-01-01
影响因子：
6.4
通讯作者：
GROSS, KC
中科院分区：
文献类型：
--
作者：
YUNOVITZ, H;GROSS, KC
未缀合的N-聚糖Manalpha 1-->6（Manalpha 1-->3）Manalpha 1-->6（Manalpha 1-->3）-Manbeta 1-->4GlcNac（Man 5GlcNAc）和Manalpha 1-->6（Manalpha 1--> 3）（Xylbeta 1-->2）-Manbeta 1--> 4GlcNAcbeta 1-->4（Fucalpha 1-->3）GlcNAc显示出刺激和延迟成熟的绿色番茄果实（Lycopersicon esculentum Mill. CV. Rutgers）分别以1和10 ng（g鲜重）-1（Priem和Gross 1992 Plant Physiol.98：399-401）。此外，10个非共轭N-聚糖的出现和结构，包括Man 5GlcNAc。最近报道了在番茄果实中的毒性（Priem等，1993，Plant Physiol.102：445-458）。在这项工作中，我们研究了Man 5GlcNAc和几种化合物之间的潜在相互作用，通过使用一个系统，允许果皮盘被保持长达14天后切除。进行研究以确定Man 5GlcNAc、吲哚-3-乙酸（IAA）的作用。伴刀豆球蛋白A和番茄凝集素对成熟的影响，如通过皮肤的红色着色所定义的。在果皮圆片中成熟。不像完整的水果。不受1 ng（g鲜重）-1 Man 5GlcNAc的影响。然而，在10 ng（g鲜重）-1时，花盘表现出延迟成熟，并且延迟着色是半乳糖依赖的。在40 μ g（g鲜重）-1半乳糖存在下，10 μ g（g鲜重）-1的Man 5GlcNAc抑制100 μ M IAA诱导的着色刺激。Man 5GlcNAc在两种凝集素的存在下失去生物活性。这些结果支持了植物生长发育过程中IAA和各种寡糖可能的相互作用，并首次表明番茄凝集素的生理意义。
The unconjugated N-glycans Manalpha1-->6(Manalpha1-->3)Manalpha1-->6(Manalpha1-->3)-Manbeta1-->4GlcNac (Man5GlcNAc) and Manalpha1-->6(Manalpha1--> 3)(Xylbeta1-->2)-Manbeta1-->4GlcNAcbeta1-->4(Fucalpha1-->3)GlcNAc were shown to stimulate and delay ripening of mature green tomato fruit (Lycopersicon esculentum Mill. cv. Rutgers) at 1 and 10 ng (g fresh weight)-1, respectively (Priem and Gross 1992 Plant Physiol. 98: 399-401). Also, the occurrence and structure of 10 unconjugated N-glycans, including Man5GlcNAc. in tomato fruit were recently reported (Priem et al. 1993, Plant Physiol. 102: 445-458). In this work, we studied the potential interaction between Man5GlcNAc and several compounds by using a system that allowed pericarp discs to be kept up to 14 days after excision. Studies were performed to determine the effect of Man5GlcNAc, indole-3-acetic acid (IAA). concanavalin A and tomato lectin on ripening as defined by red coloration of the skin. Ripening in pericarp discs. unlike that in intact fruit. was unaffected by 1 ng (g fresh weight)-1 Man5GlcNAc. However, discs showed delayed ripening with 10 ng (g fresh weight)-1, and the delay of coloration was galactose dependent. Man5GlcNAc at 10 na (g fresh weight)-1 inhibited the stimulation of coloration induced by 100 muM IAA in the presence of 40 mug (g fresh weight)-1 galactose. Man5GlcNAc lost biological activity in the presence of the two lectins. These results support the possible interaction of IAA and various oligosaccharides during plant growth and development, and for the first time suggest a physiological significance for tomato lectin.