HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family
HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family
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DOI：
10.1105/tpc.006999
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发表时间：
2003-02-01
期刊：
影响因子：
11.6
通讯作者：
Roby, D
中科院分区：
文献类型：
--
作者：
Balagué, C;Lin, BQ;Roby, D
植物的过敏反应（HR）是一种程序性细胞死亡，通常与抗病性有关。拟南芥中的一种新突变hlm 1，其引起细胞死亡的异常调节，表现为病变模拟表型和改变的HR，分离为单个隐性等位基因。广谱的防御机制仍然功能或组成的突变体植物，这也表现出增加的抗性的一个有毒菌株的假单胞菌pv番茄。在相同病原体的无毒菌株的反应中，hlm 1突变体表现出不同的能力来限制细菌的生长，这取决于病原体表达的无毒基因。HLM 1基因编码环核苷酸门控通道CNGC 4。对爪蟾卵母细胞中HLM 1/CNGC 4基因产物的初步研究（由内而外膜片钳技术）表明，CNGC 4对K+和Na+都是可渗透的，并被cGMP和cAMP激活。HLM 1基因的表达受病原体感染和某些病原体相关信号的诱导。因此，HLM 1可能构成导致HR/耐药的信号通路的共同下游组分。
The hypersensitive response (HR) in plants is a programmed cell death that is commonly associated with disease resistance. A novel mutation in Arabidopsis, hlm1, which causes aberrant regulation of cell death, manifested by a lesion-mimic phenotype and an altered HR, segregated as a single recessive allele. Broad-spectrum defense mechanisms remained functional or were constitutive in the mutant plants, which also exhibited increased resistance to a virulent strain of Pseudomonas syringae pv tomato. In response to avirulent strains of the same pathogen, the hlm1 mutant showed differential abilities to restrict bacterial growth, depending on the avirulence gene expressed by the pathogen. The HLM1 gene encodes a cyclic nucleotide-gated channel, CNGC4. Preliminary study of the HLM1/CNGC4 gene product in Xenopus oocytes (inside-out patch-clamp technique) showed that CNGC4 is permeable to both K+ and Na+ and is activated by both cGMP and cAMP. HLM1 gene expression is induced in response to pathogen infection and some pathogen-related signals. Thus, HLM1 might constitute a common downstream component of the signaling pathways leading to HR/resistance.