Bigger Isn't Always Better: Bulking Up Impedes Receptor Internalization.
Bigger Isn't Always Better: Bulking Up Impedes Receptor Internalization.
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DOI：
10.1016/j.bpj.2018.01.037
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发表时间：
2018-03
影响因子：
3.4
通讯作者：
A. Kenworthy
中科院分区：
文献类型：
--
作者：
A. Kenworthy
The process of endocytosis is critically important for controlling a wide range of biological processes, including cell signaling. In recent years, much emphasis has been placed on uncovering mechanisms responsible for bending membranes at endocytic sites, elucidating the role of individual proteins in different stages of endocytosis, and determining how scission of endocytic vesicles is accomplished (1–3). However, much less is known about how physical factors such as the size of endocytic cargo impacts their uptake by this process. This is an especially interesting question for the case of membrane receptors, which serve as binding sites for ligands of varying sizes. Previous studies have suggested that the steric bulk of receptors can influence their internalization (4, 5), but the biophysical basis for this effect is unclear. Here, DeGroot et al. rigorously test how receptor size controls internalization by directly visualizing the incorporation of engineered receptor-ligand complexes of various sizes into clathrin-coated structures at the cell surface (6).
