Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices.
Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices.
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DOI：
10.1038/nrg.2017.15
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
Weitz DA
中科院分区：
文献类型：
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作者：
Prakadan SM;Shalek AK;Weitz DA
细胞分析的最新进展已经证明了细胞行为的实质性异质性，一旦被认为是“相同的”，挑战细胞“类型”和“状态”的基本概念。毫不奇怪，这些发现引起了人们对深入表征细胞表型之间的多样性、相互关系和可塑性的浓厚兴趣。为了探索这些问题，需要能够广泛且可控地分析许多单个细胞的实验平台。在这里，微流体结构-无论是基于阀，液滴还是基于微滴-都具有重要作用，因为它们可以促进单细胞及其组分的简单捕获和处理，相对于传统的基于板的方法减少劳动力和成本，同时还提高一致性。在本文中，我们回顾了当前与哺乳动物单细胞“组学”微流体相关的最新方法，并讨论了挑战和未来的机遇。
Recent advances in cellular profiling have demonstrated substantial heterogeneity in the behaviour of cells once deemed ‘identical’, challenging fundamental notions of cell ‘type’ and ‘state’. Not surprisingly, these findings have elicited substantial interest in deeply characterizing the diversity, interrelationships and plasticity among cellular phenotypes. To explore these questions, experimental platforms are needed that can extensively and controllably profile many individual cells. Here, microfluidic structures—whether valve-, droplet- or nanowell-based—have an important role because they can facilitate easy capture and processing of single cells and their components, reducing labour and costs relative to conventional plate-based methods while also improving consistency. In this article, we review the current state-of-the-art methodologies with respect to microfluidics for mammalian single-cell ‘omics’ and discuss challenges and future opportunities.