Methods, Challenges and Potentials of Single Cell RNA-seq.
Methods, Challenges and Potentials of Single Cell RNA-seq.
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DOI：
10.3390/biology1030658
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发表时间：
2012-11-16
期刊：
影响因子：
4.2
通讯作者：
Hebenstreit D
中科院分区：
文献类型：
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作者：
Hebenstreit D
RNA测序（RNA-seq）已成为转录组学的首选工具。最近的几项研究表明，它成功地适应单细胞分析。这使得新的生物学见解细胞分化，细胞间变异和基因调控，以及这些方面如何相互依赖。在这里，我回顾了目前的单细胞RNA-seq（scRNA-seq）的努力，并讨论了实验方案，挑战和潜力。
RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.