Proteomic responses in Arabidopsis thaliana seedlings treated with ethylene
Proteomic responses in Arabidopsis thaliana seedlings treated with ethylene
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DOI：
10.1039/c1mb05159h
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Cooper, Bret
中科院分区：
文献类型：
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作者：
Chen, Ruiqiang;Binder, Brad M.;Cooper, Bret
乙烯 (ET) 是一种挥发性激素，可调节果实成熟、植物生长、发育和应激反应。通过拟南芥基因解剖鉴定出的 ET 信号通路的关键组成部分包括 5 个 ET 受体、负调节因子 CTR1 和正调节因子 EIN2，所有这些都定位于内质网。这些蛋白质之间的信号传导机制仍未解决，ET 反应的目标尚不完全清楚。因此，我们使用质谱法来鉴定来自在环境空气中保存或用 ET 处理 3 小时的黄化拟南芥幼苗的微粒体膜制剂中的蛋白质。我们比较了 ET 暴露幼苗和对照幼苗的 3814 种蛋白质，并鉴定了 304 种蛋白质具有显着的积累变化。积累增加的蛋白质与ET生物合成、细胞形态发生、氧化应激和囊泡分泌有关，而积累减少的蛋白质是核糖体蛋白质和受油菜素类固醇（另一种参与细胞伸长的激素）正向调节的蛋白质。包括 EIN2 在内的几种蛋白质在 ET 处理后似乎出现了差异性磷酸化，这表明这些蛋白质的活性或稳定性可能受到磷酸化的控制。 TUA3 是微管的一个组成部分，有助于细胞形态变化，在 ET 处理后表现出积累增加和差异磷酸化。为了验证 TUA3 在 ET 反应中的作用，对 tua3 突变体进行了评估。突变幼苗改变了与 ET 相关的生长运动。数据表明，ET 感知导致蛋白质组的快速变化，并且这些变化是信号传导和发育的重要组成部分。这些数据是通过系统生物学探索 ET 信号传导的基础。
Ethylene (ET) is a volatile hormone that modulates fruit ripening, plant growth, development and stress responses. Key components of the ET-signaling pathway identified by genetic dissection in Arabidopsis thaliana include five ET receptors, the negative regulator CTR1 and the positive regulator EIN2, all of which localize to the endoplasmic reticulum. Mechanisms of signaling among these proteins are still unresolved and targets of ET responses are not fully known. So, we used mass spectrometry to identify proteins in microsomal membrane preparations from etiolated A. thaliana seedlings maintained in ambient air or treated with ET for 3 h. We compared 3814 proteins from ET-exposed seedlings and controls and identified 304 proteins with significant accumulation changes. The proteins with increased accumulation were involved in ET biosynthesis, cell morphogenesis, oxidative stress and vesicle secretion while those with decreased accumulation were ribosomal proteins and proteins positively regulated by brassinosteroid, another hormone involved in cell elongation. Several proteins, including EIN2, appeared to be differentially phosphorylated upon ET treatment, which suggests that the activity or stability of these proteins may be controlled by phosphorylation. TUA3, a component of microtubules that contributes to cellular morphological change, exhibited both increased accumulation and differential phosphorylation upon ET treatment. To verify the role of TUA3 in the ET response, tua3 mutants were evaluated. Mutant seedlings had altered ET-associated growth movements. The data indicate that ET perception leads to rapid proteomic change and that these changes are an important part of signaling and development. The data serve as a foundation for exploring ET signaling through systems biology.