Acute inhibition of glial cells in the NTS does not affect respiratory and sympathetic activities in rats exposed to chronic intermittent hypoxia
Acute inhibition of glial cells in the NTS does not affect respiratory and sympathetic activities in rats exposed to chronic intermittent hypoxia
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DOI：
10.1016/j.brainres.2012.12.003
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发表时间：
2013-02-16
期刊：
影响因子：
2.9
通讯作者：
Machado, Benedito H.
中科院分区：
文献类型：
--
作者：
Costa, Kaue M.;Moraes, Davi J. A.;Machado, Benedito H.
近年来的研究表明，神经元-神经胶质相互作用参与了神经元活动调控的多个方面。在孤束核（NTS）中，神经元-胶质细胞的相互作用被认为参与了对生理挑战的自主神经反应的整合。然而，NTS神经胶质细胞是否可能影响呼吸和心血管控制，以及它们是否可能是缺氧挑战下自主神经和呼吸反应的组成部分，仍有待证实。在这里，我们研究了NTS胶质细胞是否在中枢呼吸和交感神经活动的调节中发挥强直性作用，以及在暴露于慢性间歇性缺氧（CIH）诱导的呼吸-交感神经偶联的变化中发挥强直性作用，CIH是中枢自主神经和呼吸可塑性的模型。注入NTS的尾侧和中间亚核没有改变幼年大鼠原位制备的基线呼吸和交感参数。在先前暴露于CIH的大鼠中观察到类似的结果。同样，cih诱导的呼吸-交感偶联变化不受fct介导的抑制影响。然而，将FCt显微注射到腹侧髓质后，呼吸频率发生了变化。我们的研究结果表明，急性神经胶质抑制NTS不影响基线呼吸和交感神经控制。此外，我们得出结论，NTS胶质细胞可能不是持续表现交感和呼吸适应CIH所必需的。我们的工作提供了NTS中神经元-胶质细胞相互作用不参与基线呼吸和交感神经控制的证据。(c) 2012 Elsevier B.V.版权所有
Recent studies suggest that neuron-glia interactions are involved in multiple aspects of neuronal activity regulation. In the nucleus tractus solitarius (NTS) neuron-glia interactions are thought to participate in the integration of autonomic responses to physiological challenges. However, it remains to be shown whether NTS glial cells might influence breathing and cardiovascular control, and also if they could be integral to the autonomic and respiratory responses to hypoxic challenges. Here, we investigated whether NTS glia play a tonic role in the modulation of central respiratory and sympathetic activities as well as in the changes in respiratory-sympathetic coupling induced by exposure to chronic intermittent hypoxia (CIH), a model of central autonomic and respiratory plasticity We show that bilateral microinjections of fluorocitrate (FCt), a glial cell inhibitor, into the caudal and intermediate subnuclei of the NTS did not alter baseline respiratory and sympathetic parameters in in situ preparations of juvenile rats. Similar results were observed in rats previously exposed to CIH. Likewise, CIH-induced changes in respiratory-sympathetic coupling were unaffected by FCt-mediated inhibition. However, microinjection of FCt into the ventral medulla produced changes in respiratory frequency. Our results show that acute glial inhibition in the NTS does not affect baseline respiratory and sympathetic control. Additionally, we conclude that NTS glial cells may not be necessary for the continuous manifestation of sympathetic and respiratory adaptations to CIH. Our work provides evidence that neuron-glia interactions in the NTS do not participate in baseline respiratory and sympathetic control. (c) 2012 Elsevier B.V. All rights reserved.