Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis
Exogenous sucrose influences KEA1 and KEA2 to regulate abscisic acid-mediated primary root growth in Arabidopsis
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外源蔗糖影响 KEA1 和 KEA2 调节拟南芥脱落酸介导的初生根生长
DOI：
10.1016/j.plantsci.2022.111209
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发表时间：
2022
期刊：
影响因子：
5.2
通讯作者：
Tengguo Zhang
中科院分区：
文献类型：
--
作者：
Sheng Zheng;Min Su;Zhongfei Shi;Haixia Gao;Cheng Ma;Shan Zhu;Lina Zhang;Guofan Wu;Wangze Wu;Juan Wang;Jinping Zhang;Tengguo Zhang
拟南芥K+-外排逆向转运蛋白(KEA)1和KEA2是叶绿体内包膜K+/H+逆向转运蛋白，在叶绿体发育和幼苗生长中起重要作用。然而，KEA1和KEA2在幼苗早期发育过程中的作用还知之甚少。在本工作中，我们发现在拟南芥中，KEA1和KEA2通过调节光合作用和ABA信号通路来调节初生根的生长。表型分析表明，在不含蔗糖的情况下，kea1kea2突变体的初生根长度显著短于野生型哥伦比亚0(Col-0)植株。然而，这一表型可以通过外施蔗糖来补救。同时，HPLC-MS/MS结果表明，在无蔗糖培养条件下，突变体kea1kea2的ABA积累显著低于Col-0。转录组分析表明，许多与ABA信号有关的关键基因在kea1kea2突变体中受到抑制。我们认为，KEA1和KEA2的缺乏不仅影响光合作用，而且可能通过ABA依赖的方式参与初生根的生长。本研究证实了KEA1和KEA2在影响初生根生长中的新功能。
Arabidopsis K+-efflux antiporter (KEA)1 and KEA2 are chloroplast inner envelope membrane K+/H+antiporters that play an important role in plastid development and seedling growth. However, the function of KEA1 and KEA2 during early seedling development is poorly understood. In this work, we found that in Arabidopsis, KEA1 and KEA2 mediated primary root growth by regulating photosynthesis and the ABA signaling pathway. Phenotypic analyses revealed that in the absence of sucrose, the primary root length of thekea1kea2mutant was significantly shorter than that of the wild-type Columbia-0 (Col-0) plant. However, this phenotype could be remedied by the external application of sucrose. Meanwhile, HPLC–MS/MS results showed that in sucrose-free medium, ABA accumulation in thekea1kea2mutant was considerably lower than that in Col-0. Transcriptome analysis revealed that many key genes involved in ABA signals were repressed in thekea1kea2mutant. We concluded that KEA1 and KEA2 deficiency not only affected photosynthesis but was also involved in primary root growth likely through an ABA-dependent manner. This study confirmed the new function of KEA1 and KEA2 in affecting primary root growth.