Allergen-induced cytokine phenotypes in mice:: role of CD4+ and CD8+ T cell populations
Allergen-induced cytokine phenotypes in mice:: role of CD4+ and CD8+ T cell populations
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DOI：
10.1111/j.1365-2222.2005.02209.x
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发表时间：
2005-04-01
影响因子：
6.1
通讯作者：
Kimber, I
中科院分区：
文献类型：
--
作者：
Dearman, RJ;Humphreys, N;Kimber, I
背景表达2型细胞因子的CD4(+) T细胞与高分子量过敏原引起的哮喘发病机制有关。 BALB/c 品系小鼠局部暴露于低分子量化学接触和呼吸道过敏原分别刺激 1 型和 2 型细胞因子分泌表型。 目的 检测细胞因子阳性 CD4(+) 和 CD8(+) T 细胞的相对频率及其对这些细胞因子分泌谱的贡献。 方法 雌性 BALB/c 品系小鼠开始局部暴露于化学过敏原或载体后 13 天，分离引流耳廓淋巴结独自一人。通过流式细胞术计数细胞内细胞因子（IL-4和IFN-γ）阳性CD4（+）和CD8（+）淋巴细胞的频率。通过阴性选择评估CD4(+)和CD8(+)细胞对细胞因子分泌谱的相对贡献。结果暴露于过敏原导致IFN-γ(+)CD4(+)和CD8(+)淋巴细胞的频率增加，尽管偏苯三酸酐(TMA)和2,4-二硝基氯苯(DNCB)激活的淋巴结细胞之间没有显着差异。 TMA 处理诱导的 IL-4(+) CD4(+) 细胞数量大约是 DNCB 处理的五倍。在另外一对接触性和呼吸道过敏原中也观察到了这种细胞因子染色模式；分别为福尔马林和异硫氰酸荧光素。 结论 这些数据表明，不同类别的化学过敏原在小鼠中诱导的不同免疫反应与 IFN-γ(+) 细胞频率的变化无关，但与表达 IL-4 的 CD4(+) T 细胞的差异频率有关。
Background CD4(+) T cells expressing type 2 cytokines have been implicated in the pathogenesis of asthma to high-molecular-weight allergens. Topical exposure of BALB/c strain mice to low-molecular-weight chemical contact and respiratory allergens stimulates type 1 and type 2 cytokine secretion phenotypes, respectively.Objective To examine the relative frequencies of cytokine-positive CD4(+) and CD8(+) T cells and their contributions to these cytokine secretion profiles.Methods Draining auricular lymph nodes were isolated 13 days after initiation of topical exposure of female BALB/c strain mice to chemical allergen, or to vehicle alone. The frequency of intracellular cytokine (IL-4 and IFN-gamma)-positive CD4(+) and CD8(+) lymphocytes was enumerated by flow cytometry. The relative contribution of CD4(+) and CD8(+) cells to cytokine secretion profiles was assessed by negative selection.Results Exposure to allergen resulted in an increased frequency of both IFN-gamma(+) CD4(+) and CD8(+) lymphocytes, although there were no marked differences between trimellitic anhydride (TMA)- and 2,4-dinitrochlorobenzene (DNCB)-activated lymph node cells. Treatment with TMA induced approximately five times as many IL-4(+) CD4(+) cells as did exposure to DNCB. This pattern of cytokine staining was also observed for a further pair of contact and respiratory allergens; respectively, formalin and fluorescein isothiocyanate.Conclusion These data demonstrate that the divergent immune responses induced in mice by different classes of chemical allergen are independent of changes in the frequency of IFN-gamma(+) cells, but are associated with differential frequencies of IL-4-expressing CD4(+) T cells.