Structure and flexibility of nanoscale protein cages designed by symmetric self-assembly.
Structure and flexibility of nanoscale protein cages designed by symmetric self-assembly.
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DOI：
10.1021/ja402277f
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发表时间：
2013-05-22
影响因子：
15
通讯作者：
Yeates, Todd O.
中科院分区：
文献类型：
--
作者：
Lai, Yen-Ting;Tsai, Kuang-Lei;Sawaya, Michael R.;Asturias, Francisco J.;Yeates, Todd O.
设计自组装成复杂结构的蛋白质分子是纳米生物技术领域的一个突出目标。这样做的一种设计策略涉及将两种天然蛋白质遗传融合在一起，已知每种天然蛋白质都能自身形成简单的寡聚体（例如二聚体或三聚体）。如果两个这样的组件可以融合在一个几何预定义的配置，该设计的子单元可以，原则上，组装成高度对称的架构。初步实验表明，一个12亚基四面体笼，直径16 nm，可以构建以下这样的程序。在这里，我们表征以这种方式构建的蛋白质笼的多个晶体结构，包括从相同的基本蛋白质亚基的两种突变形式组装的笼。所设计的组件的灵活性和它们的偏离目标模型进行了描述，沿着与进一步的设计开发的影响。
Designing protein molecules that self-assemble into complex architectures is an outstanding goal in the area of nanobiotechnology. One design strategy for doing this involves genetically fusing together two natural proteins, each of which is known to form a simple oligomer on its own (e.g. a dimer or trimer). If two such components can be fused in a geometrically predefined configuration, that designed subunit can, in principle, assemble into highly symmetric architectures. Initial experiments showed that a 12-subunit tetrahedral cage, 16 nm in diameter, could be constructed following such a procedure. Here we characterize multiple crystal structures of protein cages constructed in this way, including cages assembled from two mutant forms of the same basic protein subunit. The flexibilities of the designed assemblies and their deviations from the target model are described, along with implications for further design developments.
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DOI：
10.1073/pnas.1112595109
发表时间：
2012-05-08
影响因子：
11.1
作者：
Lanci, Christopher J.;MacDermaid, Christopher M.;Saven, Jeffery G.
通讯作者：
Saven, Jeffery G.
DOI：
10.1126/science.1219364
发表时间：
2012-06-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
King NP;Sheffler W;Sawaya MR;Vollmar BS;Sumida JP;André I;Gonen T;Yeates TO;Baker D
通讯作者：
Baker D
影响因子：
3.7
作者：
Harris, A;Forouhar, F;Luo, M
通讯作者：
Luo, M
影响因子：
3.4
作者：
Sweeney, Blake;Zhang, Tiequan;Schwartz, Russell
通讯作者：
Schwartz, Russell
DOI：
10.1016/j.str.2011.12.007
发表时间：
2012-02-08
期刊：
Structure (London, England : 1993)
影响因子：
--
作者：
Yang Z;Fang J;Chittuluru J;Asturias FJ;Penczek PA
通讯作者：
Penczek PA