Reusable Standardized Universal Interface Module (RSUIM) for Generic Organ-on-a-Chip Applications
Reusable Standardized Universal Interface Module (RSUIM) for Generic Organ-on-a-Chip Applications
复制标题
适用于通用芯片器官应用的可重复使用标准化通用接口模块 (RSUIM)
DOI：
10.3390/mi10120849
复制
发表时间：
2019
期刊：
影响因子：
3.4
通讯作者：
Xiaolin Wang
中科院分区：
文献类型：
--
作者：
Qiyue Sun;Jianghua Pei;Qinyu Li;Kai Niu;Xiaolin Wang
基于模块化的多器官芯片能够实现更稳定和灵活的配置，以更好地模拟复杂的生物现象，用于多功能生物医学应用。然而，现有的基于磁性的互连模式主要是通过将磁体直接嵌入和/或固定到模块化微流体装置中来实现的，仅供一次性使用，这将不可避免地增加制造过程中的复杂性和成本。在这里，我们提出了一种新的设计，一个可重复使用的标准化通用接口模块（RSUIM），这是非常适合通用的器官芯片应用程序和它们集成到多器官系统。基于粘贴和基于夹持的互连模式都是以即插即用的方式开发的，没有流体泄漏。此外，由于模块化设计的灵活性，通过并行配置将多个组装的模块化装置集成到高通量平台中是简单的。为了验证其有效性，将通用血管化器官芯片模块与基于粘贴的RSUIM相结合，进行了微血管网络和血管化肿瘤模型的构建实验，并对其重复性和抗癌药物筛选验证进行了定量分析。我们相信，这种RSUIM设计将成为广泛的器官芯片应用的标准和关键配件，并且易于以低成本商业化。
The modular-based multi-organ-on-a-chip enables more stable and flexible configuration to better mimic the complex biological phenomena for versatile biomedical applications. However, the existing magnetic-based interconnection modes are mainly realized by directly embedding and/or fixing magnets into the modular microfluidic devices for single use only, which will inevitably increase the complexity and cost during the manufacturing process. Here, we present a novel design of a reusable standardized universal interface module (RSUIM), which is highly suitable for generic organ-on-chip applications and their integration into multi-organ systems. Both pasting-based and clamping-based interconnection modes are developed in a plug-and-play manner without fluidic leakage. Furthermore, due to the flexibility of the modular design, it is simple to integrate multiple assembled modular devices through parallel configuration into a high throughput platform. To test its effectiveness, experiments on the construction of both the microvascular network and vascularized tumor model are performed by using the integration of the generic vascularized organ-on-a-chip module and pasting-based RSUIM, and their quantitative analysis results on the reproducibility and anti-cancer drug screening validation are further performed. We believe that this RSUIM design will become a standard and critical accessory for a broad range of organ-on-a-chip applications and is easy for commercialization with low cost.
影响因子：
4.6
作者：
Mathur A;Loskill P;Shao K;Huebsch N;Hong S;Marcus SG;Marks N;Mandegar M;Conklin BR;Lee LP;Healy KE
通讯作者：
Healy KE
影响因子：
6.1
作者：
Wang X;Phan DT;Sobrino A;George SC;Hughes CC;Lee AP
通讯作者：
Lee AP
影响因子：
6.1
作者：
Phan DTT;Wang X;Craver BM;Sobrino A;Zhao D;Chen JC;Lee LYN;George SC;Lee AP;Hughes CCW
通讯作者：
Hughes CCW