Ultrasensitive electrochemical DNA sensor for virulence invA gene of Salmonella using silver nanoclusters as signal probe
Ultrasensitive electrochemical DNA sensor for virulence invA gene of Salmonella using silver nanoclusters as signal probe
复制标题
以银纳米簇为信号探针检测沙门氏菌毒力invA基因的超灵敏电化学DNA传感器
DOI：
10.1016/j.snb.2018.05.133
复制
发表时间：
2018-11-01
影响因子：
8.4
通讯作者：
Zheng, Haisong
中科院分区：
文献类型：
--
作者：
Ye, Yongkang;Liu, Yaqian;Zheng, Haisong
本工作建立了一种检测沙门氏菌invA基因序列的灵敏电化学传感方法，该方法将直接信号诱导剂银纳米团簇（AgNCs）和富含胱氨酸（C-rich）的信号探针DNA组装金纳米颗粒（sDNA-AuNPs）相结合。将巯基化捕获探针共价固定在玻碳电极上，并与靶DNA序列（tDNA）和sDNA-AuNPs进一步杂交，形成一种双螺旋结构。该结构中富含C的sDNA可作为AgNCs的模板，在检测tDNA的过程中产生直接的伏安信号。在最佳条件下，该传感器在1 ~ 0.1 nM范围内具有良好的线性，检测限为0.162 fM（S/N = 3）。该方法具有良好的选择性、稳定性和重复性，可用于检测沙门氏菌invA基因的PCR产物。
This work described a sensitive electrochemical sensing method for detection of invA gene sequence of Salmonella by integrating direct signal inducer of silver nanoclusters (AgNCs) and cystine-rich (C-rich) signal probe DNA assembled gold nanoparticls (sDNA-AuNPs). Thiolated capture probe was covalently immobilized onto the glassy carbon electrode, and further hybridized with target DNA sequence (tDNA) and sDNA-AuNPs onto form a sandwich-type structure. The C-rich sDNA in the structure was then used as template for the formation of AgNCs, which can produce the direct voltametric signal during the detection of tDNA. Under optimal conditions, the fabricated biosensor showed good linearity from 1 fM to 0.1 nM with a detection limit of 0.162 fM (S/N = 3). It exhibited good selectivity, stability and reproducibility and can be applied to detect Salmonella effectively by detecting the polymerase chain reaction products of invA gene.