Antioxidant and antimutagenic activities of pomegranate peel extracts
Antioxidant and antimutagenic activities of pomegranate peel extracts
复制标题
DOI：
10.1016/s0308-8146(02)00279-0
复制
发表时间：
2003-03-01
期刊：
影响因子：
8.8
通讯作者：
Jena, BS
中科院分区：
文献类型：
--
作者：
Negi, PS;Jayaprakasha, GK;Jena, BS
石榴果皮粉碎后，在索氏萃取器中用乙酸乙酯(EtOAc)、丙酮、甲醇和水提取4h。通过磷钼络合物的形成和Ames试验测定其对叠氮化钠致突变性的抑制作用。果皮提取物均表现出明显的抗氧化能力，但水提取物的抗氧化能力最低。由于在每种溶剂中的四种浓度(25、50、75和100微克/毫升)的反应不同，抗氧化能力的大小顺序也不同。各提取物对鼠伤寒沙门氏菌(TA100和TA1535)的叠氮钠致突变性均有不同程度的抑制作用。在2500杯/皿条件下，所有提取物均表现出较强的抗突变作用。水提物的抗突变作用次之，其次是丙酮、乙酸乙酯和甲醇提物。结果表明，石榴皮提取物具有抗氧化和抗突变双重作用，可作为生物防腐剂应用于食品和医药领域。(C)2002爱思唯尔科学有限公司，版权所有。
dDried pomegranate peels were powdered and extracted in a Soxhlet extractor with ethyl acetate (EtOAc), acetone, methanol and water for 4 h each. The dried extracts were used to determine their antioxidant capacity by the formation of phosphomolybdenum complex and antimutagenicity against the mutagenicity of sodium azide by the Ames test. All the peel extracts exhibited marked antioxidant capacity, but the water extract was the lowest. The order of antioxidant capacity varied because of differential responses at four concentrations (25, 50, 75 and 100 mug/ml) in each solvent. All the extracts decreased sodium azide mutagenicity in Salmonella typhimurium strains (TA100 and TA1535), either weakly or strongly. At 2500 mug/plate all the extracts showed strong antimutagenicity. The antimutagenicity of the water extract was followed by acetone, EtOAc and methanol extracts. The overall results showed that the pomegranate peel extracts have both antioxidant and antimutagenic properties and may be exploited as biopreservatives in food applications and nentraceuticals. (C) 2002 Elsevier Science Ltd, All rights reserved.