Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats
Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats
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星形胶质细胞谷氨酸-谷氨酰胺循环参与大鼠炎症伤害感受的调节
DOI：
10.3969/j.issn.1673-5374.2011.35.004
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Bin Geng
中科院分区：
文献类型：
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作者：
Tiancheng Wang;Jing Wang;Yayi Xia;Hongyu Guo;Haili Shen;Bin Geng
我们以前的行为学研究表明星形胶质细胞谷氨酸-谷氨酰胺循环参与了福尔马林诱导的脊髓中枢敏化过程，但很少有形态学证据。本研究采用免疫荧光双标记技术，发现大鼠鞘内注射PBS和足底注射福尔马林后，胶质细胞酸性蛋白（GFAP）和谷氨酰胺合成酶（GS）的表达增加，GFAP/GS共表达改变到第III层和第IV层。鞘内注射GS特异性抑制剂甲硫氨酸亚砜亚胺后，福尔马林诱导的脊髓背角GFAP和GS表达和共表达的变化受到抑制。星形胶质细胞的形态、分布和数量均恢复到正常水平。鞘内注射谷氨酰胺可逆转甲硫氨酸亚砜亚胺的抑制作用。星形胶质细胞表现出显着的激活和分布扩展到第V和VI层。本研究为星形胶质细胞谷氨酸-谷氨酰胺循环参与福尔马林诱导的脊髓中枢敏化提供了形态学证据。
Our previous behavioral studies have indicated that the astroglial glutamate-glutamine cycle is involved in the process of formalin-induced spinal cord central sensitization, but there was little morphological evidence. In this study, double-labeling immunofluorescence techniques showed that after rats were intrathecally injected with PBS and plantarly injected with formalin, glial fibrillary acidic protein (GFAP) and glutamine synthesase (GS) expression were increased and GFAP/GS coexpression was changed to include layers III and IV. After intrathecal injection of methionine sulfoximine, a GS specific inhibitor, the formalin-induced change in expression and coexpression of GFAP and GS in spinal cord dorsal horns was inhibited. The morphology, distribution and quantity of astrocytes recovered to normal levels. An intrathecal glutamine injection reversed the inhibitory effect of methionine sulfoximine. Astrocytes showed significant activation and distribution extended to layers V and VI. The present study provides morphological evidence that the astroglial glutamate-glutamine cycle is involved in the process of formalin-induced spinal cord central sensitization.