Non-cytotoxic Silver Nanoparticle-Polysaccharide Nanocomposites with Antimicrobial Activity
Non-cytotoxic Silver Nanoparticle-Polysaccharide Nanocomposites with Antimicrobial Activity
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DOI：
10.1021/bm900039x
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发表时间：
2009-06-01
影响因子：
6.2
通讯作者：
Paoletti, Sergio
中科院分区：
文献类型：
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作者：
Travan, Andrea;Pelillo, Chiara;Paoletti, Sergio
在这项工作中，我们研究（i）的形成和稳定的银纳米粒子的生物活性壳聚糖衍生的多糖溶液中，（ii）的抗菌性能，无论是在溶液中或在三维水凝胶结构，通过混合物与多糖藻酸盐，和（iii）后者的纳米复合材料对不同的真核细胞系的细胞毒性。抗菌结果表明，这些纳米复合材料系统显示出非常有效的杀菌活性，对革兰氏+和革兰氏-细菌。然而，水凝胶对三种不同的真核细胞系没有显示出任何细胞毒性作用。这是由于固定在凝胶基质中的纳米颗粒可以通过与细菌膜的简单接触来发挥其抗微生物活性，而它们不能被真核细胞摄取和内化。这一新的发现可以有利地有助于响应对纳米颗粒的毒性的日益增长的关注，并促进在生物材料的制备中使用银-生物聚合物复合材料。
In this work we study (i) the formation and stabilization of silver nanoparticles in a bioactive chitosan-derived polysaccharide solution, (ii) the antimicrobial properties, either in solution or in 3D hydrogel structures, obtained by mixtures with the polysaccharide alginate, and (iii) the cytotoxicity of the latter nanocomposite materials on different eukaryotic cell lines. Antimicrobial results show that these nanocomposite systems display a very effective bactericidal activity toward both Gram+ and Gram- bacteria. However, the hydrogel does not show any cytotoxic effect toward three different eukaryotic cell lines. This is due to the fact that the nanoparticles, immobilized in the gel matrix, can exert their antimicrobial activity by simple contact with the bacterial membrane, while they can not be uptaken and internalized by eukaryotic cells. This novel finding could advantageously contribute to responding to the growing concerns on the toxicity of nanoparticles and facilitate the use of silver-biopolymer composites in the preparation of biomaterials.