Profiling the chlorogenic acids of aster by HPLC-MSn
Profiling the chlorogenic acids of aster by HPLC-MSn
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DOI：
10.1002/pca.935
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发表时间：
2006-11-01
影响因子：
3.3
通讯作者：
Kuhnert, Nikolai
中科院分区：
文献类型：
--
作者：
Clifford, Michael N.;Zheng, Wang;Kuhnert, Nikolai
用HPLC-MS法从三尖瓣紫菀的水醇提取物中鉴定出33种绿原酸。花蕾这些是对香豆酰奎尼酸、咖啡酰奎尼酸、阿魏酰奎尼酸、二咖啡酰奎尼酸和二阿魏酰奎尼酸各三种异构体，以及对香豆酰-咖啡酰奎尼酸、对香豆酰-阿魏酰奎尼酸和咖啡酰阿魏酰奎尼酸各六种异构体。在菊科植物中仅发现咖啡酰奎宁酸和二咖啡酰奎宁酸。6种对香豆酰-阿魏酰奎宁酸中的3种（3-阿魏酰-4-对香豆酰奎宁酸、3-阿魏酰-5-对香豆酰奎宁酸和4-阿魏酰-5-对香豆酰奎宁酸）以前在自然界中未观察到。顺式-5-对香豆酰奎尼酸被鉴定为浓度约为更常见的反式异构体的25%。阿魏酰奎宁酸和二阿魏酰奎宁酸分别占单酰基和二酰基绿原酸级分的主导地位，使这种植物材料成为这些商业上不可获得的物质的有用来源。这33种绿原酸在芦荟的叶和茎中均未检测到。ageratoides Turcz.，或在A.三尖菊变种Gerla或A.马兰在黄芪根中只检测到阿魏酰奎宁酸。三尖菊不可能检测到任何1-酰基绿原酸、任何具有琥珀酸取代基的绿原酸或任何基于粘奎尼酸的绿原酸。版权所有（c）2006约翰威利父子有限公司。
Using HPLC-MS', 33 chlorogenic acids were identified in an aqueous-alcoholic extract of Aster ageratoides Turcz. flower buds. These were three isomers each of p-coumaroylquinic acid, caffeoylquinic acid, feruloylquinic acid, dicaffeoylquinic acid and difer-uloylquinic acid, and six isomers each of p-coumaroyl-caffeoylquinic acid, p-coumaroyl-feruloylquinic acid and caffeoylferuloylquinic acid. Only the caffeoylquinic acids and dicaffeoylquinic acids have been reported previously in Asteraceae. Three of the six p-coumaroyl-feruloylquinic acids (3-feruloyl-4-p-coumaroylquinic acid, '3-feruloyl-5-p-coumaroylquinic acid and 4-feruloyl-5-p-coumaroylquinic acid) have not been observed previously in nature. Cis-5-p-coumaroylquinic acid was identified at a concentration ca 25% that of the more common trans isomer. The feruloylquinic acids and diferuloylquinic acids dominated the mono- and di-acyl chlorogenic acid fractions, respectively, making this plant material a useful source of these commercially nonavailable substances. These 33 chlorogenic acids were not detected in the leaves or stem of A. ageratoides Turcz., or in the flower buds of A. ageratoides Turcz. var. Gerla or A. kalimeris indica (L) Sch. Bip. Only the feruloylquinic acids were detected in the root of A. ageratoides Turcz. It was not possible to detect any 1-acyl chlorogenic acids, any chlorogenic acids with a succinic acid substituent, or any chlorogenic acids based on muco-quinic acid. Copyright (c) 2006 John Wiley & Sons, Ltd.