AMELIORATION OF THE PHYSIOLOGICAL AND BIOCHEMICAL-CHANGES OF ACUTE-PANCREATITIS USING AN ANTI-TNF-ALPHA POLYCLONAL ANTIBODY
AMELIORATION OF THE PHYSIOLOGICAL AND BIOCHEMICAL-CHANGES OF ACUTE-PANCREATITIS USING AN ANTI-TNF-ALPHA POLYCLONAL ANTIBODY
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DOI：
10.1016/0002-9610(94)90076-0
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发表时间：
1994-01-01
影响因子：
3
通讯作者：
GABER, AO
中科院分区：
文献类型：
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作者：
GREWAL, HP;ELDIN, AM;GABER, AO
肿瘤坏死因子（TNF）是一种炎性细胞因子，可能是重症急性胰腺炎相关全身性后遗症发生的重要介质。本研究的目的是确定用多克隆抗体中和TNF-α是否可以改善大鼠模型中重症胰腺炎的选定生化参数。通过将人工胆汁顺行注射到胆管中诱发胰腺炎。将40只大鼠随机分为4组：不手术组（对照组）、胆管盐水输注组（假手术组）、胰腺炎动物安慰剂治疗组（安慰剂+ Pr）和胰腺炎动物多克隆抗体（PAb + Pr）预处理组。在胆管输注后2小时测量血清TNF-α、淀粉酶、钙、红细胞压积、葡萄糖和腹水量。与用安慰剂治疗的胰腺炎动物相比，用PAb预治疗产生了所有参数的显著改善（p < 0.001）。此外，在胰腺炎动物中升高的TNF-α在治疗动物中显著降低（p < 0.001）。这些结果表明，TNF-α可能是一个重要的介质在重症急性胰腺炎的全身表现的演变。
Tumor necrosis factor (TNF) is an inflammatory cytokine that may be an important mediator in the development of the systemic sequelae associated with severe acute pancreatitis. The purpose of this study was to determine whether the neutralization of TNF-alpha with a polyclonal antibody could ameliorate selected biochemical parameters of severe pancreatitis in a rat model. Pancreatitis was induced by an antegrade injection of artificial bile into the bile duct. Forty rats were randomized into 4 groups: no surgery (controls),saline infusion to bile duct (sham), placebo treatment in animals with pancreatitis (placebo + Pr), and pretreatment with a polyclonal antibody (PAb) in animals with pancreatitis (PAb + Pr). Serum TNF-alpha, amylase, calcium, hematocrit, glucose, and ascites volume were measured 2 hours after bile duct infusion. Pretreatment with the PAb produced a significant improvement in all parameters when compared with pancreatitis animals treated with placebo (p < 0.001). In addition, TNF-alpha, which was elevated in animals with pancreatitis, was reduced significantly in treated animals (p < 0.001). These results suggest that TNF-alpha may be an important mediator in the evolution of the systemic manifestations of severe acute pancreatitis.