Immunohistochemical investigation of the presence and distribution of cementum-associated lipopolysaccharides in periodontal disease.
Immunohistochemical investigation of the presence and distribution of cementum-associated lipopolysaccharides in periodontal disease.
复制标题
牙周病中牙骨质相关脂多糖的存在和分布的免疫组织化学研究。
DOI：
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发表时间：
1986
影响因子：
3.5
通讯作者：
F. Smales
中科院分区：
文献类型：
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作者：
F. Hughes;F. Smales
应用免疫组织化学方法检测了14颗因牙周病拔除的人牙中牙骨质相关脂多糖（LPS）的分布。针对结合到环氧活化的Sepharose 6 B-多粘菌素B柱的口服LPS类型的制备物获得自体亲和纯化的抗LPS抗体。G类免疫球蛋白的浓度。通过火箭免疫电泳测定制备的血清中的M和A，并使用ELISA检查血清对LPS制备物的反应性。制备牙齿的研磨切片，并与制备的自体抗血清一起孵育，然后通过兔PAP系统检测IG结合。LPS被检测到的牙骨质已被事先暴露于牙周袋，并扩展到以前的水平的结缔组织附着在1毫米内。LPS仅在牙骨质表面被检测到，并且在任何情况下均未观察到LPS渗透到牙骨质表面下。这些结果表明，在根面平整术中，为了使根面不含细菌内毒素，不需要广泛去除牙骨质。
An immunohistochemical technique was used to investigate the distribution of cementum-associated LPS in 14 human teeth extracted because of periodontal disease. Autologous affinity purified anti-LPS antibodies were obtained against a preparation of oral LPS types bound to an epoxy-activated Sepharose 6B-polymyxin B column. The concentration of immunoglobulin classes G. M and A in the prepared sera was assayed by rocket immunoelectrophoresis and the reactivity of the sera was checked using an ELISA to the LPS preparation. Ground sections of the teeth were prepared and incubated with prepared autologous antiserum, followed by detection of Ig binding by a rabbit PAP system. LPS was detected on cementum which had been previously exposed to a periodontal pocket and extended to within I mm of the previous level of connective tissue attachment. LPS was only detected on the surface of cementum and on no occasion was LPS seen penetrating into sub-surface cementum. These results suggest that extensive removal of sub-surface cementum is not necessary in order to render root surfaces free of bacterial endotoxins during root planing procedures.