BACTERIOHOPANETETROL - ABUNDANT LIPID IN FRANKIA CELLS AND IN NITROGEN-FIXING NODULE TISSUE
BACTERIOHOPANETETROL - ABUNDANT LIPID IN FRANKIA CELLS AND IN NITROGEN-FIXING NODULE TISSUE
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DOI：
10.1104/pp.95.1.111
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发表时间：
1991-01-01
期刊：
影响因子：
7.4
通讯作者：
JONES, AD
中科院分区：
文献类型：
--
作者：
BERRY, AM;MOREAU, RA;JONES, AD
在Alnus和Ceanothus的结节组织中发现了一类不寻常的具有两亲性的类脂。在Frankia spp的溶剂提取物中检测到较高水平的相同类脂(占细胞总类脂的20-50%)。细胞。然而，寄主根中没有这种脂类。用高效液相色谱/火焰离子化检测器对脂类进行纯化和定量。苯酚-硫酸测定和质子核磁共振分析表明，纯化后的脂类不是糖脂。主要物种的质谱图与已发表的细菌酮(C35H62O4)的谱图一致，这是一种五环三萜类化合物。
An unusual class of lipid with amphiphilic properties has been detected in nodule tissue of Alnus and Ceanothus. High levels of the same lipid (20-50% of total cell lipids) were detected in solvent extracts of Frankia spp. cells. However, the lipid was absent in host roots. The lipid was purified and quantified by high-performance liquid chromatography/flame ionization detector. Phenol-sulfuric acid determinations and proton nuclear magnetic resonance indicated that the purified lipid is not a glycolipid. Mass spectra of the predominant species are consistent with published spectra for bacteriohopanetetrol (C35H62O4), a pentacyclic triterpenoid, or hopanoid.