Late asthmatic reactions induced by inhalation of allergen-derived T cell peptides
Late asthmatic reactions induced by inhalation of allergen-derived T cell peptides
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DOI：
10.1164/rccm.200305-690oc
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发表时间：
2004-01-01
影响因子：
24.7
通讯作者：
Kay, AB
中科院分区：
文献类型：
--
作者：
Ali, FR;Oldfield, WLG;Kay, AB
在患有特应性和哮喘的个体中，皮内注射过敏原衍生的T细胞肽诱导孤立的晚期哮喘反应（LARs），随后是支气管对肽的低反应性，抑制过敏原诱导的皮肤晚期反应，以及体外改变的T细胞功能。实验室动物数据表明，如果吸入肽，也会发生“活化”和“耐受性”。在这项研究中，我们表明，吸入Fel d 1衍生肽诱导孤立LAR的个人哮喘敏感的猫过敏原与先前证明的皮内注射。LARS伴有嗜酸性粒细胞增多和痰液中总半胱氨酰白三烯的非显著性升高。与皮内途径不同，重复吸入肽与LAIR的消除无关，并产生与首次暴露相当的痰嗜酸性粒细胞增多症。此外，没有抑制皮肤的后期反应，整个猫皮屑。因此，由吸入的变应原衍生肽诱导的孤立LAIR代表了一种新的激发哮喘模型，并且与皮肤或肺中低反应性（“耐受性”）的诱导无关。
In individuals with atopy and asthma, allergen-derived T cell peptides injected intradermally induce isolated late asthmatic reactions (LARs) followed by bronchial hypo responsiveness to peptide, inhibition of the allergen-induced cutaneous late-phase reaction, and altered T cell function in vitro. Laboratory animal data indicate that "activation" and "tolerance" also occur if peptides are inhaled. In this study, we show that inhalation of Fel d 1-derived peptides induced isolated LAR in individuals with asthma sensitive to cat allergen comparable with that previously demonstrated using intradermal injection. LARS were accompanied by eosinophilia and nonsignificant elevations of total cysteinyl leukotrienes in the sputum. Unlike the intradermal route, repeated inhalation of peptides was not associated with abrogation of the LAIR and produced a sputum eosinophilia comparable with the first exposure. In addition, there was no inhibition of the cutaneous late-phase reaction to whole cat dander. Thus, isolated LAIR induced by inhaled, allergen-derived peptides represent a novel model of provoked asthma and are not associated with the induction of hyporesponsiveness ("tolerance") in the skin or lung.