Identification of glycogen in electron micrographs of thin tissue sections.
Identification of glycogen in electron micrographs of thin tissue sections.
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DOI：
10.1083/jcb.8.3.575
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发表时间：
1960-12
期刊：
影响因子：
--
通讯作者：
FAWCETT, D W
中科院分区：
文献类型：
--
作者：
REVEL, J P;NAPOLITANO, L;FAWCETT, D W
对几种动物器官中糖原的电子显微镜形态进行了研究。糖原几乎总是以直径在150到400埃之间的圆形颗粒出现。糖原的固有电子密度因组织而异；然而，沃森所描述的氢氧化铅处理会使颗粒深度染色。用离心法从所研究的一些组织中分离出糖原颗粒。根据化学和组织化学标准，这种小球被证明是糖原。当在电子显微镜下观察小球的薄片时，可以看到它们由密集排列的颗粒组成，类似于在完整组织中发现的颗粒。通过短期接触氢氧化铅，这种小球也会在电子显微镜下染色。
The electron microscopic appearance of glycogen has been studied in the organs of several animal species. Glycogen almost always appears as roughly circular granules from 150 to 400 A in diameter. The intrinsic electron density of glycogen varies from tissue to tissue; however, treatment with lead hydroxide as described by Watson deeply stains the granules. Glycogen pellets were isolated from some of the tissues studied by centrifugation. Such pellets were shown to be glycogen by chemical and histochemical criteria. When thin sections of the pellet are examined under the electron microscope they can be seen to consist of densely packed granules similar to those found in the intact tissues. Such pellets are also stained for electron microscopy by short exposure to lead hydroxide.