Sizing subcellular organelles and nanoparticles confined within aqueous droplete
Sizing subcellular organelles and nanoparticles confined within aqueous droplete
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DOI：
10.1021/ac8000385
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发表时间：
2008-05-01
影响因子：
7.4
通讯作者：
Chiu, Daniel T.
中科院分区：
文献类型：
--
作者：
Gadd, Jennifer C.;Kuyper, Christopher L.;Chiu, Daniel T.
Ibis的文章描述了两种互补的技术，单粒子跟踪和相关光谱，用于精确测量皮升体积的水液滴中的纳米粒子。单粒子跟踪可以很好地与明亮的粒子，可以连续照明和成像，我们证明了这种方法的大小单荧光珠。荧光相关光谱检测小强度的突发从颗粒或分子扩散通过共聚焦探针体积，这很好地与暗淡和快速扩散的颗粒或分子，我们证明了FCS的大小突触囊泡限制在水滴。结合液滴操作和分析的最新进展，我们预计这种在游离溶液中确定单个纳米颗粒和分子大小的能力将补充现有的探测细胞系统，亚细胞器和纳米颗粒的工具。
Ibis article describes two complementary techniques, single-particle tracking and correlation spectroscopy, for accurately sizing nanoparticles confined within picoliter volume aqueous droplets. Single-particle tracking works well with bright particles that can be continuously illuminated and imaged, and we demonstrated this approach for sizing single fluorescent beads. Fluorescence correlation spectroscopy detects small intensity bursts from particles or molecules diffusing through the confocal probe volume, which works well with dim and rapidly diffusing particles or molecules; we demonstrated FCS for sizing synaptic vesicles confined in aqueous droplets. In combination with recent advances in droplet manipulations and analysis, we anticipate this capability to size single nanoparticles and molecules in free solution will complement existing tools for probing cellular systems, subcellular organelles, and nanoparticles.