Biofilm formation by a Bacillus subtilis strain that produces gamma-polyglutamate.
Biofilm formation by a Bacillus subtilis strain that produces gamma-polyglutamate.
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DOI：
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发表时间：
2006
期刊：
影响因子：
1.5
通讯作者：
M. Morikawa;Shinji Kagihiro;M. Haruki;K. Takano;S. Branda;R. Kolter;S. Kanaya
中科院分区：
文献类型：
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作者：
M. Morikawa;Shinji Kagihiro;M. Haruki;K. Takano;S. Branda;R. Kolter;S. Kanaya
枯草芽孢杆菌B-1是一种能形成稳定的漂浮生物膜的环境菌株，其产生的细胞外基质经纯化，并确定主要由γ-聚谷氨酸（γ-PGA）组成，分子量超过1，000 kDa。生物膜形成和B产生γ-PGA。枯草芽孢杆菌B-1的活性随Mn（2+）或甘油浓度的增加而增加。γ-PGA在静置培养中以生长相关的方式产生，并形成漂浮生物膜。然而，γ-PGA在振荡培养条件下以非生长相关的方式产生。当B.枯草杆菌B-1在微曝气培养系统中生长，漂浮生物膜的形成和γ-PGA的产生显著延迟，表明氧耗尽参与了静置培养中漂浮生物膜形成的初始步骤。膜蛋白质组学分析表明，鞭毛蛋白，寡肽通透酶和Vpr蛋白酶前体是由细胞在漂浮生物膜和菌落产生的主要蛋白质。
The extracellular matrix produced by Bacillus subtilis B-1, an environmental strain that forms robust floating biofilms, was purified, and determined to be composed predominantly of gamma-polyglutamate (gamma-PGA), with a molecular mass of over 1,000 kDa. Both biofilm formation and gamma-PGA production by B. subtilis B-1 increased with increasing Mn(2+) or glycerol concentration. gamma-PGA was produced in a growth-associated manner in standing culture, and floating biofilms were formed. However, gamma-PGA was produced in a non-growth-associated manner in shaking culture conditions. When B. subtilis B-1 was grown in a microaerated culture system, floating biofilm formation and gamma-PGA production were significantly retarded, suggesting that oxygen depletion is involved in the initial steps of floating biofilm formation in standing culture. Proteomic analysis of membrane proteins demonstrated that flagellin, oligopeptide permease and Vpr protease precursor were the major proteins produced by cells in a floating biofilm and a colony.