Amperometric detection of stimulus-induced quantal release of catecholamines from cultured superior cervical ganglion neurons.
Amperometric detection of stimulus-induced quantal release of catecholamines from cultured superior cervical ganglion neurons.
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DOI：
10.1073/pnas.92.15.6938
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发表时间：
1995-07
影响因子：
11.1
通讯作者：
Zhuan Zhou;S. Misler
中科院分区：
文献类型：
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作者：
Zhuan Zhou;S. Misler
电流法已被用于实时电化学检测从嗜铬细胞和肥大细胞的分泌颗粒中释放的儿茶酚胺和吲哚胺。使用改进的灵敏度碳纤维电极，我们现在报告的检测量子儿茶酚胺释放在表面的胞体的新生儿上级颈神经节神经元，布满轴突静脉曲张含有突触囊泡。局部应用含有高K+或黑寡妇蜘蛛毒液的浴溶液，其中每一种都大大增强了突触处递质的自发量子释放，诱发了小振幅（2-20 pA）、短持续时间（0.5-2 ms）的电流计量量子“尖峰”的冲击。中位尖峰电荷计算为11.3 fC。这个数字对应于每释放量子3.5 × 10（4）个儿茶酚胺分子，或约1%，由嗜铬颗粒内容物的放电引起。
Amperometry has been used for real-time electrochemical detection of the quantal release of catecholamines and indolamines from secretory granules in chromaffin and mast cells. Using improved-sensitivity carbon fiber electrodes, we now report the detection of quantal catecholamine release at the surface of somas of neonatal superior cervical ganglion neurons that are studded with axon varicosities containing synaptic vesicles. Local application of a bath solution containing high K+ or black widow spider venom, each of which greatly enhances spontaneous quantal release of transmitter at synapses, evoked barrages of small-amplitude (2-20 pA), short-duration (0.5-2 ms) amperometric quantal "spikes". The median spike charge was calculated as 11.3 fC. This figure corresponds to 3.5 x 10(4) catecholamine molecules per quantum of release, or approximately 1% that evoked by the discharge of the contents of a chromaffin granule.