Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
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DOI：
10.2807/1560-7917.es.2017.22.28.30573
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发表时间：
2017-07-13
期刊：
影响因子：
19
通讯作者：
Angen, O.
中科院分区：
文献类型：
--
作者：
Larsen, J.;Sunde, M.;Angen, O.
我们评估了一种广泛使用的基于培养的方法，用于检测从丹麦和挪威的猪和猪圈内环境中收集的样品中家畜相关的耐甲氧西林金黄色葡萄球菌（LA-MRSA）。在含有头孢西丁和氨曲南的胰蛋白酶豆汤中选择性富集导致假阴性结果的比例很高（26%;57/221）。在此基础上，我们建议重新考虑在动物和环境样品中使用选择性富集检测LA-MRSA。
We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.