2 HUMAN GLUTAMATE DECARBOXYLASES, 65-KDA GAD AND 67-KDA GAD, ARE EACH ENCODED BY A SINGLE GENE
2 HUMAN GLUTAMATE DECARBOXYLASES, 65-KDA GAD AND 67-KDA GAD, ARE EACH ENCODED BY A SINGLE GENE
复制标题
DOI：
10.1073/pnas.89.6.2115
复制
发表时间：
1992-03-15
影响因子：
11.1
通讯作者：
TOBIN, AJ
中科院分区：
文献类型：
--
作者：
BU, DF;ERLANDER, MG;TOBIN, AJ
我们报告了编码两种人谷氨酸脱羧酶（GAD; L-谷氨酸1-羧基裂解酶，EC 4.1.1.15）GAD 65和GAD 67的cDNA的分离和测序。人GAD 65 cDNA编码M（r）65，000多肽，具有585个氨基酸残基，而人GAD 67编码M（r）67，000多肽，具有594个氨基酸残基。两种cDNA都指导细菌表达系统中酶活性GAD的合成。每个cDNA与单一种类的脑mRNA杂交，并与人类基因组DNA中的一组特定的限制性片段杂交。荧光标记的GAD探针与人染色体的原位杂交将人GAD 65基因定位于染色体10p11.23，将人GAD 67基因定位于染色体2 q31。我们得出结论，GAD 65和GAD 67各自来自一个单独的基因。我们描述的cDNA应该允许细菌生产用于诊断和预测胰岛素依赖型糖尿病的测试抗原。
We report the isolation and sequencing of cDNAs encoding two human glutamate decarboxylases (GADs; L-glutamate 1-carboxy-lyase, EC 4.1.1.15), GAD65 and GAD67. Human GAD65 cDNA encodes a M(r) 65,000 polypeptide, with 585 amino acid residues, whereas human GAD67 encodes a M(r) 67,000 polypeptide, with 594 amino acid residues. Both cDNAs direct the synthesis of enzymatically active GADs in bacterial expression systems. Each cDNA hybridizes to a single species of brain mRNA and to a specific set of restriction fragments in human genomic DNA. In situ hybridization of fluorescently labeled GAD probes to human chromosomes localizes the human GAD65 gene to chromosome 10p11.23 and the human GAD67 gene to chromosome 2q31. We conclude that GAD65 and GAD67 each derive from a single separate gene. The cDNAs we describe should allow the bacterial production of test antigens for the diagnosis and prediction of insulin-dependent diabetes mellitus.