EXPRESSION OF HEAT SHOCK PROTEIN 70 IN RABBIT LIVER AFTER CONTRAST-ENHANCED ULTRASOUND AND RADIOFREQUENCY ABLATION
EXPRESSION OF HEAT SHOCK PROTEIN 70 IN RABBIT LIVER AFTER CONTRAST-ENHANCED ULTRASOUND AND RADIOFREQUENCY ABLATION
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DOI：
10.1016/j.ultrasmedbio.2009.08.001
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发表时间：
2010-01-01
影响因子：
2.9
通讯作者：
Imai, Yasuharu
中科院分区：
文献类型：
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作者：
Liu, Guang-Jian;Moriyasu, Fuminori;Imai, Yasuharu
热消融治疗诱导的热休克蛋白（HSP）可能有助于将肿瘤抗原呈递给宿主免疫系统，并成为肝癌消融治疗的重要佐剂。本文描述了我们对超声造影（CEUS）和射频（RF）消融后兔肝脏中HSP70表达的初步研究。 25只雄性新西兰大白兔分为5组：对照组（n=5）、超声组（n=5）、CEUS组（n=5）、RF组（n=5）和CEUS+RF组（n=5）。本实验使用临床超声和射频消融设备。 Sonazoid 用作造影剂。术后24小时处死所有动物，通过免疫组化染色和Western blot分析检测HSP70。在未进行射频消融的各组中，对照组和超声组肝组织中均无HSP70表达，而CEUS组肝组织中检测到HSP70阳性表达，平均光密度为0.33。 RF组和CEUS+RF组中，远离消融区域的正常肝组织中也有细胞表达HSP70。 RF组中HSP70表达的平均密度为0.31，CEUS+RF组中HSP70表达的平均密度分别为0.35。 RF组和CEUS+RF组的HSP70表达分布，边缘区域强于距边缘1cm的肝组织，且消融组织中未见HSP70表达的证据。 RF组边缘区域HSP70表达的平均密度为0.47，CEUS+RF组边缘区域HSP70表达的平均密度分别为0.42。 CEUS 检查和射频消融后，使用 Sonazoid 进行的 CEUS 可能会在正常肝实质中产生 HSP70 表达。 （电子邮件：moriyasu@tokyo-med.ac.jp） (C) 2010 年世界超声医学与生物学联合会。
Heat shock proteins (HSPs) induced by thermal ablation therapy may help presenting tumor antigen to the host immune system and be a valuable adjuvant in the ablation therapy of liver cancer. This paper described our preliminary study on the expression of HSP70 in rabbit liver after contrast-enhanced ultrasound (CEUS) and radiofrequency (RF) ablation. Twenty-five male New Zealand white rabbits were divided into five groups as: control group (n=5), ultrasound group (n=5), CEUS group (n=5), RF group (n=5) and CEUS + RF group (n=5). Clinical ultrasound and RF ablation equipment were used in the present experiment. Sonazoid was used as the contrast agent. All the animals were sacrificed 24 h after the procedure, and HSP70 was detected by immunohistochemistry staining and Western blot analysis. In the groups without RF ablation, there was no evidence of HSP70 expression in the liver tissue of the control group and ultrasound group, whereas positive HSP70 expression was detected in the liver tissue of the CEUS group, with a mean optical density of 0.33. In the RF and CEUS + RF groups, there were cells showing HSP70 expression in the normal liver tissue far from the ablation region. The mean densities of HSP70 expression were 0.31 in the RF group and 0.35 in the CEUS + RF group, respectively. With regard to the distribution of HSP70 expression of the RF and CEUS + RF groups, the marginal areas were stronger than liver tissue 1 cm away from the margin, and the ablated tissues showed no evidence of HSP70 expression. The mean density of HSP70 expression in the marginal areas were 0.47 in the RF group and 0.42 in the CEUS + RF group, respectively. CEUS using Sonazoid may produce HSP70 expression in the normal liver parenchyma after CEUS examination and RF ablation. (E-mail: moriyasu@tokyo-med.ac.jp) (C) 2010 World Federation for Ultrasound in Medicine & Biology.