A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction
A rodent model of myocardial infarction for testing the efficacy of cells and polymers for myocardial reconstruction
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DOI：
10.1038/nprot.2006.188
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发表时间：
2006-01-01
期刊：
影响因子：
14.8
通讯作者：
Lee, Randall J.
中科院分区：
文献类型：
--
作者：
Huang, Ngan F.;Sievers, Richard E.;Lee, Randall J.
我们已经建立了一个强大的心肌梗死（MI）大鼠模型。在这里，我们描述了一步一步的协议，建立缺血再灌注大鼠模型的MI。我们还描述了如何将间充质干细胞（MSC）与纤维蛋白一起注射治疗，以显示该模型的应用。此外，为了确认梗死中纤维蛋白和细胞的存在，还描述了通过组织学技术对MSC和纤维蛋白的可视化。缺血-再灌注MI模型可以被修改和推广用于各种可注射聚合物、细胞类型、药物、DNA及其组合。该模型可以在7天或更短的时间内创建，这取决于治疗干预的时间。
We have developed a robust rat model of myocardial infarction (MI). Here we describe the step-by-step protocol for creating an ischemia-reperfusion rat model of MI. We also describe how to deliver therapeutic injections of mesenchymal stem cells (MSCs) together with fibrin, to show an application of this model. In addition, to confirm the presence of fibrin and cells in the infarct, visualization of MSCs and fibrin by histological techniques are also described. The ischemia-reperfusion MI model can be modified and generalized for use with various injectable polymers, cell types, drugs, DNA and combinations thereof. The model can be created in 7 days or less, depending on the timing of therapeutic intervention.