Fabrication and Characterization of a Glucose-sensitive Antibacterial Chitosan-Polyethylene Oxide Hydrogel.
Fabrication and Characterization of a Glucose-sensitive Antibacterial Chitosan-Polyethylene Oxide Hydrogel.
复制标题
葡萄糖敏感抗菌壳聚糖-聚环氧乙烷水凝胶的制备和表征
DOI：
10.1016/j.polymer.2015.11.016
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发表时间：
2016-01-15
期刊：
影响因子：
4.6
通讯作者：
Xu X
中科院分区：
文献类型：
--
作者：
Xiao Y;Gong T;Jiang Y;Wang Y;Wen ZT;Zhou S;Bao C;Xu X
通过化学交联和固定葡萄糖氧化酶（GOx），获得了一种新型葡萄糖敏感壳聚糖-聚氧化乙烯（CS/PEO =1:0.5~1:2.5）水凝胶，该水凝胶具有甲硝唑（MNZ）控释功能。通过傅立叶变换红外光谱（FTIR）、压缩机械测试、流变分析、细胞毒性测试和牙龈卟啉单胞菌抗菌测试对该水凝胶进行了表征。研究发现，CS-PEO 复合水凝胶的机械性能和生物相容性明显优于单组分水凝胶。这可能是物理交联和形成半互穿网络（semi-IPN）的结果。此外，这种新型水凝胶还具有自我调节能力，能在环境葡萄糖刺激下释放 MNZ。具体来说，它在葡萄糖浓度较高时释放更多的药物，因此抑制牙龈卟啉单胞菌的能力更强。这项研究表明，葡萄糖敏感抗菌水凝胶作为一种新的治疗材料，在治疗或预防糖尿病患者牙周炎方面具有很大的潜力。
A novel glucose-sensitive chitosan-polyethylene oxide (CS/PEO =1:0.5~1:2.5) hydrogel with controlled release of metronidazole (MNZ) was obtained by chemical cross-linking and immobilization of glucose oxidase (GOx). The hydrogel was characterized by Fourier-transformed infrared spectroscopy (FTIR), compressive mechanical test, rheological analysis, cytotoxicity test, and antibacterial test against Porphyromonas gingivalis. The study found that the CS-PEO composite hydrogel possessed significantly better mechanical properties and biocompatibility than a single-component hydrogel. This might result from the physical cross-linking and formation of semi-interpenetrating network (semi-IPN). In addition, this novel hydrogel has self-regulate ability to release MNZ in response to the environmental glucose stimulus. Specifically, it released more drugs at higher glucose concentration, thus can lead to a greater ability to inhibit Porphyromonas gingivalis. This study has demonstrated the glucose-sensitive antibacterial hydrogel has a great potential as a new therapeutic material for treatment or prevention of periodontitis in diabetic patients.