REDUCTION OF MYOCARDIAL INFARCT SIZE BY NEUTROPHIL DEPLETION - EFFECT OF DURATION OF OCCLUSION
REDUCTION OF MYOCARDIAL INFARCT SIZE BY NEUTROPHIL DEPLETION - EFFECT OF DURATION OF OCCLUSION
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DOI：
10.1016/0002-8703(86)90461-8
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发表时间：
1986-10-01
影响因子：
4.8
通讯作者：
LUCCHESI, BR
中科院分区：
文献类型：
--
作者：
JOLLY, SR;KANE, WJ;LUCCHESI, BR
在狗身上进行了实验，以检测中性粒细胞减少对回旋支冠状动脉短时间(90分钟)或长时间(4小时)闭塞所导致的最终梗塞范围的影响。用绵羊抗犬中性粒细胞血清产生中性粒细胞减少症。对照组动物接受非免疫血清。用组织病理学技术和半定量计分系统检测中性粒细胞在心肌梗死中的浸润情况。在再灌注24小时的90分钟闭塞中，中性粒细胞减少与明显较小的梗死灶的发展有关：正常组，43.2%。3.3%(n=7)与中性粒细胞减少组(26.6%)比较。危险区域的3.7%(n=10)。扫描电子显微镜。然而，在缺血4h再灌流6h的实验中，中性粒细胞耗竭的趋势并没有缩小梗塞面积，但没有达到统计学意义(46.4%)。7.2%对31.5%。+-。6.0%的风险区域，正常与中性粒细胞减少)，尽管中性粒细胞在再灌注区的渗透存在差异。观察到的最终梗死面积的差异不能归因于心肌耗氧量的差异。结果表明，冠状动脉闭塞后再灌流引起的大量心肌梗死是中性粒细胞依赖的，在决定心肌缺血后再灌流时间较长的心肌梗死的命运中似乎不那么重要。
Experiments were performed in the dog to examine the effects of neutropenia on ultimate infarct size resulting from short (90 minutes) or prolonged (4 hours) circumflex coronary artery occlusion. Sheep antiserum to canine neutrophils was used to produce neutropenia. Control animals received nonimmune serum. Neutrophil infiltration into myocardial infarcts was examined using histopathologic techniques and a semiquantitative scoring system. In 90-minute occlusions with 24-hour reperfusion, neutropenia was associated with the development of significantly smaller infarcts: normopenic group, 43.2% .+-. 3.3% (n = 7) vs. neutropenic group, 26.6% .+-. 3.7% (n = 10) of the area at risk .hivin.X .+-. SEM. However, in 4-hour occlusion with 6-hour reperfusion experiments, the tendency of neutrophil depletion to reduce infarct size did not reduce infarct size did not reach statistical significance (46.4% .+-. 7.2% vs. 31.5% .+-. 6.0% of the area at risk, normopenic vs. neutropenic) despite differences in neutrophil infiltration into the reperfused region. The observed differences in ultimate infarct size could not be attributed to differences in myocardial oxygen consumption. The results suggest that a significant amount of myocardial infarction induced by a limited duration of coronary artery occlusion followed by reperfusion is neutrophil dependent and appears to be less important in determining the fate of myocrdium subjected to more prolonged periods of ischemia followed by reperfusion.