Overview of Cell Synchronization
Overview of Cell Synchronization
复制标题
DOI：
10.1007/978-1-61779-182-6_1
复制
发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Banfalvi, Gaspar
中科院分区：
文献类型：
--
作者：
Banfalvi, Gaspar
对细胞周期调控的调控机制的研究维持了人们对细胞同步化的广泛兴趣。在同步过程中，获得了代表细胞周期不同阶段的不同亚群的细胞。这些亚群然后被用来在大分子生物合成(DNA合成、基因表达、蛋白质合成)、蛋白质磷酸化、新药开发等水平上研究周期的调节机制。虽然已经描述了几种同步方法，但科学家们对这些同步技术的汇编和最新看法是普遍感兴趣的。本章概述了细胞周期同步化的基本概念和主要标准，(2)最常用的同步化方法，如物理分离(流式细胞术、电泳法、细胞荧光法纯化)、化学阻滞法、(3)胚胎细胞同步化、(4)低温同步化、(5)细胞同步化技术比较、(6)单细胞生物体同步化，以及(7)同步化对转染的影响。
Widespread interest in cell synchronization is maintained by the studies of control mechanisms involved in cell cycle regulation. During the synchronization distinct subpopulations of cells are obtained representing different stages of the cell cycle. These subpopulations are then used to study regulatory mechanisms of the cycle at the level of macromolecular biosynthesis (DNA synthesis, gene expression, protein synthesis), protein phosphorylation, development of new drugs, etc. Although several synchronization methods have been described, it is of general interest that scientists get a compilation and an updated view of these synchronization techniques. This introductory chapter summarizes: (1) the basic concepts and principal criteria of cell cycle synchronizations, (2) the most frequently used synchronization methods, such as physical fractionation (flow cytometry, dielectrophoresis, cytofluorometric purification), chemical blockade, (3) synchronization of embryonic cells, (4) synchronization at low temperature, (5) comparison of cell synchrony techniques, (6) synchronization of unicellular organisms, and (7) the effect of synchronization on transfection.