The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis1[W][OA]
The GCR1, GPA1, PRN1, NF-Y Signal Chain Mediates Both Blue Light and Abscisic Acid Responses in Arabidopsis1[W][OA]
复制标题
DOI：
10.1104/pp.106.089904
复制
发表时间：
2007-02
期刊：
影响因子：
7.4
通讯作者：
K. Warpeha;S. Upadhyay;J. Yeh;J. Adamiak;Samuel I. Hawkins;Yevgeniya R. Lapik;M. Anderson;L. Kaufman
中科院分区：
文献类型：
--
作者：
K. Warpeha;S. Upadhyay;J. Yeh;J. Adamiak;Samuel I. Hawkins;Yevgeniya R. Lapik;M. Anderson;L. Kaufman
不同种类的生物（例如植物激素）和非生物（例如不同波长的光）信号通过特定的信号转导机制起作用，以协调高等植物的发育。虽然已经取得了很大的进展，但对于大多数蓝光或脱落酸介导的事件，尚未描述完整的信号转导链。基于来自T-DNA插入突变体和酵母（酿酒酵母）的双杂交和共沉淀试验的数据，我们报告了一个信号转导链共享的蓝光和脱落酸导致光捕获叶绿素a/b结合蛋白的表达在黄化的拟南芥（拟南芥）幼苗。该链由GCR 1（编码潜在G蛋白偶联受体的唯一拟南芥蛋白）、GPA 1（唯一拟南芥Gα亚基）、Pirin 1（PRN 1; cupin超家族含铁亚组的四个成员之一）和一个由A5、B 9和可能的C9组成的核因子Y异源三聚体组成。我们还表明，这种机制是存在于吸胀种子，其中它影响发芽率。
Different classes of biotic (e.g. plant hormones) and abiotic (e.g. different wavelengths of light) signals act through specific signal transduction mechanisms to coordinate higher plant development. While a great deal of progress has been made, full signal transduction chains have not yet been described for most blue light- or abscisic acid-mediated events. Based on data derived from T-DNA insertion mutants and yeast (Saccharomyces cerevisiae) two-hybrid and coprecipitation assays, we report a signal transduction chain shared by blue light and abscisic acid leading to light-harvesting chlorophyll a/b-binding protein expression in etiolated Arabidopsis (Arabidopsis thaliana) seedlings. The chain consists of GCR1 (the sole Arabidopsis protein coding for a potential G-protein-coupled receptor), GPA1 (the sole Arabidopsis Gα-subunit), Pirin1 (PRN1; one of four members of an iron-containing subgroup of the cupin superfamily), and a nuclear factor Y heterotrimer comprised of A5, B9, and possibly C9. We also demonstrate that this mechanism is present in imbibed seeds wherein it affects germination rate.