The effects of copper additives on the quantity and cell viability of adherent Staphylococcus epidermidis in silicone implants
The effects of copper additives on the quantity and cell viability of adherent Staphylococcus epidermidis in silicone implants
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DOI：
10.1080/08927011003629300
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发表时间：
2010-01-01
期刊：
影响因子：
2.7
通讯作者：
Buergers, Ralf
中科院分区：
文献类型：
--
作者：
Gosau, Martin;Prantl, Lukas;Buergers, Ralf
本体外研究评价了硅胶植入体中铜添加剂的抗菌作用。制备用于隆胸的标准硅胶材料样本和改性载铜硅胶样本，并在表皮葡萄球菌悬浮液中孵育（2 h，37 ℃）。在使用生物荧光测定（刃天青）定量粘附的葡萄球菌后，通过活细胞或死细胞标记结合荧光显微镜定量粘附的细菌细胞的活力。在刃天青荧光定量中，较高量的粘附S。与含铜添加剂的硅胶（2701 [2158/4153] rfu）相比，在纯硅胶（4612 [2319/7540]相对荧光单位[rfu]）上检测到表皮细胞。此外，发现纯硅胶的粘附细菌细胞量（5.07% [2.03%/8.93%]）显著高于含铜添加剂的硅胶（1.72% [1.26%/2.32%]）;（p < 0.001）。活、死染色结果表明，死S.表皮细胞在纯硅胶上的粘附率（52.1%）显著低于在含铜硅胶上的粘附率（79.7%）（P < 0.001）。体外实验表明，含铜硅橡胶材料对沙门氏菌有一定的抗菌作用。表皮载铜硅胶可以防止细菌定植，从而降低硅胶植入体的感染率。
This in vitro study evaluated the antibacterial effect of copper additives in silicone implants. Specimens of a standard silicone material used in breast augmentation and modified copper-loaded silicone specimens were prepared and incubated in a Staphylococcus epidermidis suspension (2 h, 37 degrees C). After the quantification of adhering staphylococci using a biofluorescence assay (Resazurin), the viability of the adhering bacterial cells was quantified by live or dead cell labeling in combination with fluorescence microscopy. In the Resazurin fluorometric quantification, a higher amount of adhering S. epidermidis cells was detected on pure silicone (4612 [2319/7540] relative fluorescence units [rfu]) than on silicone with copper additives (2701 [2158/4153] rfu). Additionally, a significantly higher amount of adhering bacterial cells (5.07% [2.03%/8.93%]) was found for pure silicone than for silicone with copper additives (1.72% [1.26%/2.32%]); (p < 0.001). Calculations from live or dead staining showed that the percentage of dead S. epidermidis cells adhered on pure silicone (52.1%) was significantly lower than on silicone with copper additives (79.7%); (p < 0.001). In vitro, silicone material with copper additives showed antibacterial effects against S. epidermidis. Copper-loaded silicone may prevent bacterial colonization, resulting in lower infection rates of silicone implants.