In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding.
In situ tissue regeneration using a novel tissue-engineered, small-caliber vascular graft without cell seeding.
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DOI：
10.1016/j.jtcvs.2008.02.058
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发表时间：
2008-10
期刊：
影响因子：
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通讯作者：
T. Yokota;H. Ichikawa;G. Matsumiya;T. Kuratani;T. Sakaguchi;S. Iwai;Y. Shirakawa;K. Torikai;
中科院分区：
文献类型：
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作者：
T. Yokota;H. Ichikawa;G. Matsumiya;T. Kuratani;T. Sakaguchi;S. Iwai;Y. Shirakawa;K. Torikai;
各种类型的天然和合成支架与动脉组织细胞或分化的干细胞最近引起了兴趣，作为潜在的小口径血管移植物。人们认为，具有促进自体组织再生潜力的合成移植物，而无需任何接种，将比接种移植物更实用。在本研究中，我们使用一种新型的组织工程化的可生物降解的血管移植物研究了小直径动脉的原位组织再生，该血管移植物不需要体外细胞接种。通过将胶原微海绵与可生物降解的编织聚合物管混合来制造，所述可生物降解的编织聚合物管以具有聚乙醇酸（芯）和聚-L-乳酸的双层的平纹编织图案构造。乳酸（鞘）纤维。我们将这些组织工程血管移植物双侧植入杂种狗（体重，20-25 kg）的颈动脉中。植入后未使用抗凝治疗方案。我们在植入后2、4、6和12个月处死了狗（每组n = 4），并对外植体进行组织学和生物化学评价。组织学和生物化学检查显示良好的原位组织再生与内皮细胞单层，平滑肌细胞，和重建血管壁与弹性蛋白和胶原fiber. CONCLUSION我们的研究表明，这种新型的组织工程血管移植促进原位组织再生，并不需要离体细胞接种，从而赋予更好的通畅性小口径血管假体。
OBJECTIVEVarious types of natural and synthetic scaffolds with arterial tissue cells or differentiated stem cells have recently attracted interest as potential small-caliber vascular grafts. It was thought that the synthetic graft with the potential to promote autologous tissue regeneration without any seeding would be more practical than a seeded graft. In this study, we investigated in situ tissue regeneration in small-diameter arteries using a novel tissue-engineered biodegradable vascular graft that did not require ex vivo cell seeding.METHODSSmall-caliber vascular grafts (4 mm in diameter) were fabricated by compounding a collagen microsponge with a biodegradable woven polymer tube that was constructed in a plain weave pattern with a double layer of polyglycolic acid (core) and poly-L-lactic acid (sheath) fibers. We implanted these tissue-engineered vascular grafts bilaterally into the carotid arteries of mongrel dogs (body weight, 20–25 kg). No anticoagulation regimen was used after implantation. We sacrificed the dogs 2, 4, 6, and 12 months (n = 4 in each group) after implantation and evaluated the explants histologically and biochemically.RESULTSAll of the tissue-engineered vascular grafts were patent with no signs of thrombosis or aneurysm at any time. Histologic and biochemical examinations showed excellent in situ tissue regeneration with an endothelial cell monolayer, smooth muscle cells, and a reconstructed vessel wall with elastin and collagen fibers.CONCLUSIONOur study indicated that this novel tissue-engineered vascular graft promoted in situ tissue regeneration and did not require ex vivo cell seeding, thereby conferring better patency on small-caliber vascular prostheses.