Electrorotation of liposomes: verification of dielectric multi-shell model for cells
Electrorotation of liposomes: verification of dielectric multi-shell model for cells
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DOI：
10.1016/s0005-2760(97)00092-1
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发表时间：
1997-11-15
期刊：
影响因子：
--
通讯作者：
Pethig, R
中科院分区：
文献类型：
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作者：
Chan, KL;Gascoyne, PRC;Pethig, R
多壳电介质模型的可靠性，用于描述交流电动行为的细胞，已被测试通过执行electrorotation和介电泳测量单层，oligolamellar，和多层脂质体的直径范围从5到24 μ m。采用荧光显微镜、流式细胞仪、电子自旋共振等方法对脂质体的形态和膜结构进行了表征。基于适当的介电壳模型，然后使用通用的递归算法分析了各种类型的脂质体的介电性质。通过模拟，可以分配给通过应用简单的壳模型得出的参数的置信水平进行估计。从这一点，我们证实，电旋转数据使准确的测定脂质体的最外层膜的介电性质，并提供良好的指示的复杂程度的外壳和内部隔室。我们还表明，使用足够的额外的信息，如介电电泳，电旋转数据产生独特的解决方案的脂质体样颗粒的介电参数。(C)1997年Elsevier Science B.V.
The reliability of multi-shell dielectric models, used to describe the ac electrokinetic behaviour of cells, has been tested by performing electrorotation and dielectrophoretic measurements on unilamellar, oligolamellar, and multilamellar liposomes of diameters ranging from 5 to 24 mu m. Fluorescence microscopy, flow cytometry and electron,spin resonance were used to characterise the morphology and membranes of the liposomes. The dielectric properties of the various types of liposomes, based on appropriate dielectric shell models, were then analysed using a general purpose, recursive, algorithm. Through simulations, the confidence levels that can be assigned to parameters derived through application of simple shell models are estimated. From this, we confirm that electrorotation data enable accurate determinations to be made of the dielectric properties of the outermost membrane of liposomes, and provide good indications of the level of complexity of the shells and internal compartments. We also demonstrate that, used with sufficient additional information, such as that provided by dielectrophoresis, electrorotation data yields unique solutions for the dielectric parameters of liposome-like particles. (C) 1997 Elsevier Science B.V.