Epidemics of enterovirus infection in Chungnam Korea, 2008 and 2009.
Epidemics of enterovirus infection in Chungnam Korea, 2008 and 2009.
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DOI：
10.1186/1743-422x-8-297
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发表时间：
2011-06-13
期刊：
影响因子：
4.8
通讯作者：
Park J
中科院分区：
文献类型：
--
作者：
Baek K;Yeo S;Lee B;Park K;Song J;Yu J;Rheem I;Kim J;Hwang S;Choi Y;Cheon D;Park J
在此之前，我们探讨了2005年至2006年在韩国忠南分离的肠道病毒的流行模式和分子特征。本研究将这些观察结果扩展到2008年和2009年。本研究中肠道病毒的季节性流行规律和年龄分布与以往的调查结果相似。最普遍的月份是7月：2008年为42.9%，2009年为31.9%。1岁以下儿童肠道病毒阳性率最高。对肠道病毒阳性样本进行了VP 1区序列测定，将2008年分离的肠道病毒分为10种类型在2009年，有8种类型（柯萨奇病毒A4、A16、B1、B3、埃可病毒6、7、9、11、16和30）和8种类型（柯萨奇病毒A2、A4、A5、A16、B1、B5、埃可病毒11和肠道病毒71）。2008年和2009年最流行的肠道病毒血清型分别是埃可病毒30和柯萨奇病毒B1，而埃可病毒18和埃可病毒5分别是2005年和2006年最流行的类型。比较2009年韩国流行的肠道病毒型柯萨奇病毒B1和B5与各相同血清型的参考株，通过系统发育树进行遗传分析。柯萨奇病毒B1株的序列分为四个不同的簇（A，B，C和D），具有一些时间和区域亚簇。2008年和2009年韩国柯萨奇B1病毒株大多数为D群，只有“Kor 08-CVB 1 - 001 CN”为C群。柯萨奇病毒B5株分离在五个不同的遗传组（集群A-E）的支持高自举值。2001年分离的韩国菌株属于D群，而2005年和2009年分离的韩国菌株属于E群。比较韩国柯萨奇病毒B5分离株与参考株的VP 1氨基酸序列，发现9个氨基酸序列（532、562、570、571、576-578、582、583和585）发生了氨基酸序列置换。
Previously, we explored the epidemic pattern and molecular characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006. The present study extended these observations to 2008 and 2009. In this study, enteroviruses showed similar seasonal prevalent pattern from summer to fall and age distribution to previous investigation. The most prevalent month was July: 42.9% in 2008 and 31.9% in 2009. The highest rate of enterovirus-positive samples occurred in children < 1-year-old-age. Enterovirus-positive samples were subjected to sequence determination of the VP1 region, which resolved the isolated enteroviruses into 10 types in 2008 (coxsackievirus A4, A16, B1, B3, echovirus 6, 7, 9, 11, 16, and 30) and 8 types in 2009 (coxsackievirus A2, A4, A5, A16, B1, B5, echovirus 11, and enterovirus 71). The most prevalent enterovirus serotype in 2008 and 2009 was echovirus 30 and coxsackievirus B1, respectively, whereas echovirus 18 and echovirus 5 were the most prevalent types in 2005 and 2006, respectively. Comparison of coxsackievirus B1 and B5 of prevalent enterovirus type in Korea in 2009 with reference strains of each same serotype were conducted to genetic analysis by a phylogenetic tree. The sequences of coxsackievirus B1 strains segregated into four distinct clusters (A, B, C, and D) with some temporal and regional sub-clustering. Most of Korean coxsackievirus B1 strains in 2008 and 2009 were in cluster D, while only "Kor08-CVB1-001CN" was cluster C. The coxsackievirus B5 strains segregated in five distinct genetic groups (clusters A-E) were supported by high bootstrap values. The Korean strains isolated in 2001 belonged to cluster D, whereas Korean strains isolated in 2005 and 2009 belonged to cluster E. Comparison of the VP1 amino acid sequences of the Korean coxsackievirus B5 isolates with reference strains revealed amino acid sequence substitutions at nine amino acid sequences (532, 562, 570, 571, 576-578, 582, 583, and 585).