Monitoring microbial community structure and succession of an A/O SBR during start-up period using PCR-DGGE
Monitoring microbial community structure and succession of an A/O SBR during start-up period using PCR-DGGE
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使用 PCR-DGGE 监测启动期间 A/O SBR 的微生物群落结构和演替。
DOI：
10.1016/s1001-0742(08)62255-x
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发表时间：
2009-01-01
影响因子：
6.9
通讯作者：
Ren Lijiao
中科院分区：
文献类型：
--
作者：
Wang Xiuheng;Zhang Kun;Ren Lijiao
采用聚合酶链反应(PCR)-变性梯度凝胶电泳（DGGE）技术，揭示了厌氧/好氧连续强化生物除磷（EBPR）反应器启动阶段微生物群落结构和演替规律。15d后达到较高的除磷效果，第30天除磷效率达到83.2%，完成启动。周期污泥样品DGGE图谱显示，优势菌种为19个otu（操作分类单位）。UPGMA聚类分析表明，群落演替快速与低磷去除率相关，而高磷去除率反映在稳定的群落结构上。测序结果显示，12个OTUs属于Proteobacterium、Actinobacteria、Gemmatimonadales和unaffiliate类群。变形杆菌、伸长四片菌和金缕单胞菌可能在除磷过程中起重要作用。作为已知的糖原积累菌数量很少，尚处于积累阶段的磷酸竞争候选菌对微生物群落结构的影响有限。获得顶极群落时，优势菌群为14 OTUs。未培养的细菌有Thauera sp.，未培养的γ -变形杆菌和长四片菌。
Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) protocol was employed for revealing microbial community structure and succession in a sequential anaerobic and aerobic reactor performing enhanced biological phosphorus removal (EBPR) during start-up period. High phosphorus removal was achieved after 15 d. On day 30, phosphorus removal efficiency reached to 83.2% and the start-up was finished. DGGE profiles of periodical sludge samples showed that dominant microbial species were 19 OTUs (operational taxonomy units). Unweighted pair-group method using arithmetic averages (UPGMA) clustering analysis revealed that rapid community succession correlated to low phosphorus removal rate and high phosphorus removal efficiency reflected on steady community structure. Sequencing results indicated that determined sequences (12 OTUs) belonged to Proteobacterium, Actinobacteria, Gemmatimonadales and unaffiliate group. Proteobacterium, Tetrasphaera elongate and Gernmatimonas aurantiaca may act important roles in phosphorus removal. With little amount as known glycogen accumulating organisms, Candidatus Competibacter phosphatis still at accumulating-phase had limited effect on microbial community structure. When climax community was obtained, dominant microbes were 14 OTUs. Microbes in a large amount were uncultured bacterium Thauera sp., uncultured gamma-Proteobacterium and Tetrasphaera elongata.